Zobrazeno 1 - 5
of 5
pro vyhledávání: '"Irina L. Tokmakova"'
Mu-driven transposition of recombinant mini-Mu unit DNA in the Corynebacterium glutamicum chromosome
Autor:
Sergey Vasilievich Smirnov, Irina L. Tokmakova, Natalya V. Gorshkova, Juliya S. Lobanova, Valerii Z. Akhverdyan, Sergey V. Mashko, Alexander A. Krylov
Publikováno v:
Applied Microbiology and Biotechnology
A dual-component Mu-transposition system was modified for the integration/amplification of genes in Corynebacterium. The system consists of two types of plasmids: (i) a non-replicative integrative plasmid that contains the transposing mini-Mu(LR) uni
Autor:
Elena G. Abalakina, Evgueni R. Gak, Yurgis A. V. Yomantas, Sergey V. Mashko, Svetlana M. Kazakova, Natalya V. Gorshkova, Irina L. Tokmakova
Publikováno v:
Applied and Environmental Microbiology. 76:75-83
The isolation of auxotrophic mutants, which is a prerequisite for a substantial genetic analysis and metabolic engineering of obligate methylotrophs, remains a rather complicated task. We describe a novel method of constructing mutants of the bacteri
Autor:
Irina P. Altman, Natalia Pavlovna Zakataeva, Ekaterina Aleksandrovna Kutukova, Leonid Romanovich Ptitsyn, Irina L. Tokmakova, Vitaliy Arkadyevich Livshits, Michael H. Zyiatdinov
Publikováno v:
FEBS Letters. 579:4629-4634
Overexpression of the yeaS gene encoding a protein belonging to the RhtB transporter family conferred upon cells resistance to glycyl-l-leucine, leucine analogues, several amino acids and their analogues. yeaS overexpression promoted leucine and, to
Autor:
Joanna I. Katashkina, Irina L. Tokmakova, Lubov I Golubeva, Irina G. Andreeva, Alexandra Yu Skorokhodova, Sergey V. Mashko, Tatiana M. Kuvaeva, Irina Vladimirovna Biryukova
Publikováno v:
BMC Biotechnology, Vol 7, Iss 1, p 80 (2007)
BMC Biotechnology
BMC Biotechnology
Background RSF1010 is a well-studied broad-host-range plasmid able to be mobilized to different bacteria and plants. RSF1010-derived plasmid vectors are widely used in both basic research and industrial applications. In the latter case, exploiting of
Autor:
Vitaliy Arkadyevich Livshits, Natalia Pavlovna Zakataeva, Irina L. Tokmakova, Petr Vladimirovich Troshin, Vladimir V. Aleshin
Publikováno v:
FEBS letters. 452(3)
A novel gene of Escherichia coli, rhtB, has been characterized. Amplification of this gene provides resistance to homoserine and homoserine lactone. Another E. coli gene, rhtC, provides resistance to threonine. The homologues of RhtB are widely distr