Zobrazeno 1 - 10
of 162
pro vyhledávání: '"Irina Artsimovitch"'
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-10 (2024)
Abstract After an RNA polymerase reaches a terminator, instead of dissociating from the template, it may diffuse along the DNA and recommence RNA synthesis from the previous or a different promoter. Magnetic tweezers were used to monitor such seconda
Externí odkaz:
https://doaj.org/article/251d26960a1a4c2092b959a30cc3e8e3
Autor:
Nelly Said, Mark Finazzo, Tarek Hilal, Bing Wang, Tim Luca Selinger, Daniela Gjorgjevikj, Irina Artsimovitch, Markus C. Wahl
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-18 (2024)
Abstract Transcription termination factor ρ is a hexameric, RNA-dependent NTPase that can adopt active closed-ring and inactive open-ring conformations. The Sm-like protein Rof, a homolog of the RNA chaperone Hfq, inhibits ρ-dependent termination i
Externí odkaz:
https://doaj.org/article/ef0791a398054336b30478c4f1f54dd0
Autor:
Jin Qian, Allison Cartee, Wenxuan Xu, Yan Yan, Bing Wang, Irina Artsimovitch, David Dunlap, Laura Finzi
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-11 (2024)
Abstract RNA polymerases must transit through protein roadblocks to produce full-length transcripts. Here we report real-time measurements of Escherichia coli RNA polymerase passing through different barriers. As intuitively expected, assisting force
Externí odkaz:
https://doaj.org/article/2fd007c86b294e6ba3514a48941511de
Autor:
Philipp K. Zuber, Nelly Said, Tarek Hilal, Bing Wang, Bernhard Loll, Jorge González-Higueras, César A. Ramírez-Sarmiento, Georgiy A. Belogurov, Irina Artsimovitch, Markus C. Wahl, Stefan H. Knauer
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-19 (2024)
Abstract RfaH, a paralog of the universally conserved NusG, binds to RNA polymerases (RNAP) and ribosomes to activate expression of virulence genes. In free, autoinhibited RfaH, an α-helical KOW domain sequesters the RNAP-binding site. Upon recruitm
Externí odkaz:
https://doaj.org/article/22619a1bbf0f4160960efd35b3c7e5a0
Publikováno v:
Computational and Structural Biotechnology Journal, Vol 20, Iss , Pp 5824-5837 (2022)
Metamorphic proteins constitute unexpected paradigms of the protein folding problem, as their sequences encode two alternative folds, which reversibly interconvert within biologically relevant timescales to trigger different cellular responses. Once
Externí odkaz:
https://doaj.org/article/1175cf41a2444c9196ef60a457dc980c
Autor:
Hao-Hong Pei, Tarek Hilal, Zhuo A. Chen, Yong-Heng Huang, Yuan Gao, Nelly Said, Bernhard Loll, Juri Rappsilber, Georgiy A. Belogurov, Irina Artsimovitch, Markus C. Wahl
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-14 (2020)
The bacterial helicase-like transcription factor HelD interacts with the RNA polymerase (RNAP) and together with the RNAP δ subunit enhances RNAP cycling. Here, the authors present the cryo-EM structures of the monomeric and dimeric Bacillus subtili
Externí odkaz:
https://doaj.org/article/862de4900b514335855420b64fe9f8ae
Autor:
Bing Wang, Vladimir Svetlov, Yuri I. Wolf, Eugene V. Koonin, Evgeny Nudler, Irina Artsimovitch
Publikováno v:
mBio, Vol 12, Iss 3 (2021)
In vitro
Externí odkaz:
https://doaj.org/article/8b8e001bcecf4dad84862b7335c6b922
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-13 (2019)
The antitermination factor RfaH adopts two functional states where its C-terminal domain is folded either as an α-helical hairpin or β-barrel. Here the authors employ solution state NMR measurements to show that the C-terminal domain transforms int
Externí odkaz:
https://doaj.org/article/daefa0dcbec247efbb406bb9ab9188b3
Publikováno v:
Molecules, Vol 27, Iss 12, p 3815 (2022)
The severity of the COVID-19 pandemic and the pace of its global spread have motivated researchers to opt for repurposing existing drugs against SARS-CoV-2 rather than discover or develop novel ones. For reasons of speed, throughput, and cost-effecti
Externí odkaz:
https://doaj.org/article/c9931350cf1f44f294c48c3463505e70
Autor:
Bing Wang, Irina Artsimovitch
Publikováno v:
Frontiers in Microbiology, Vol 11 (2021)
Timely and accurate RNA synthesis depends on accessory proteins that instruct RNA polymerase (RNAP) where and when to start and stop transcription. Among thousands of transcription factors, NusG/Spt5 stand out as the only universally conserved family
Externí odkaz:
https://doaj.org/article/c935ebc914eb42ad89fb3f32f54386a2