Zobrazeno 1 - 9 of 9 pro vyhledávání: '"Iresha D. Herath"'
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Synthesis of fluorinated leucines, valines and alanines for use in protein NMR
Autor: Ansis Maleckis, Elwy H. Abdelkader, Iresha D. Herath, Gottfried Otting
Publikováno v: Organic & Biomolecular Chemistry. 20:2424-2432
19F-NMR spectra of the protein GB1 produced by cell-free protein synthesis with fluorinated amino acids substituting their canonical parent amino acids show exceptionally large chemical shift dispersions, which indicate structural conservation.
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3dbaaa07374c73d299624f4771275710
https://doi.org/10.1039/d2ob00145d
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3
Localising individual atoms of tryptophan side chains in the metallo-β-lactamase IMP-1 by pseudocontact shifts from paramagnetic lanthanoid tags at multiple sites
Autor: Bim Graham, Shereen Jabar, Gottfried Otting, Ansis Maleckis, Iresha D. Herath, Colum Breen, Lydia Topping, Henry W. Orton, Stephen J. Butler, Monika Szabo
Publikováno v: Magnetic Resonance, Vol 3, Pp 1-13 (2022)
The metallo-β-lactamase IMP-1 features a flexible loop near the active site that assumes different conformations in single crystal structures, which may assist in substrate binding and enzymatic activity. To probe the position of this loop, we label
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a4e07493092ebaaf7d240872bbc958f9
https://mr.copernicus.org/articles/3/1/2022/mr-3-1-2022.pdf
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4
A Chiral Lanthanide Tag for Stable and Rigid Attachment to Single Cysteine Residues in Proteins for NMR, EPR and Time‐Resolved Luminescence Studies
Autor: Gottfried Otting, Thomas R. Berki, Iresha D. Herath, Sarah H. Hewitt, Charlotte A. Dodson, Colum Breen, Nicholas Cox, Shereen Jabar, Stephen J. Butler, Martyna Judd, Ahmad F. Kassir
Publikováno v: Herath, I D, Breen, C, Hewitt, S H, Berki, T R, Kassir, A F, Dodson, C, Judd, M, Jabar, S, Cox, N, Otting, G & Butler, S J 2021, ' A chiral lanthanide tag for stable and rigid attachment to single cysteine residues in proteins for NMR, EPR and time-resolved luminescence studies ', Chemistry-A European Journal . https://doi.org/10.1002/chem.202101143
Chemistry (Weinheim an Der Bergstrasse, Germany)
A lanthanide‐binding tag site‐specifically attached to a protein presents a tool to probe the protein by multiple spectroscopic techniques, including nuclear magnetic resonance, electron paramagnetic resonance and time‐resolved luminescence spe
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eca7463272775609da11a7debd7a4cd7
https://doi.org/10.1002/chem.202101143
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5
Synthesis of 13C/19F/2H labeled indoles for use as tryptophan precursors for protein NMR spectroscopy
Autor: Gottfried Otting, Iresha D. Herath, Ansis Maleckis
Publikováno v: Organic & Biomolecular Chemistry. 19:5133-5147
Synthesis of indoles labeled with 13C–1H and 13C–19F spin pairs is described. All syntheses utilize inexpensive carbon–13C dioxide as the 13C isotope source. Ruthenium-mediated ring-closing metathesis is the key step in construction of the 13C
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::33ac675db15dc8f786dcf31a30510907
https://doi.org/10.1039/d1ob00611h
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6
Site-Specific Incorporation of 7-Fluoro-L-tryptophan into Proteins by Genetic Encoding to Monitor Ligand Binding by
Autor: Haocheng, Qianzhu, Elwy H, Abdelkader, Iresha D, Herath, Gottfried, Otting, Thomas, Huber
Publikováno v: ACS sensors. 7(1)
A mutant aminoacyl-tRNA synthetase identified by a library selection system affords site-specific incorporation of 7-fluoro-L-tryptophan in response to an amber stop codon. The enzyme allows the production of proteins with a single hydrogen atom repl
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::cadd1035f3a2a45d2d98335d8255f64b
https://pubmed.ncbi.nlm.nih.gov/35005899
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8
Synthesis of
Autor: Ansis, Maleckis, Iresha D, Herath, Gottfried, Otting
Publikováno v: Organicbiomolecular chemistry. 19(23)
Synthesis of indoles labeled with 13C-1H and 13C-19F spin pairs is described. All syntheses utilize inexpensive carbon-13C dioxide as the 13C isotope source. Ruthenium-mediated ring-closing metathesis is the key step in construction of the 13C contai
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::d44ba6a3ee50874e6b67b49292073156
https://pubmed.ncbi.nlm.nih.gov/34032255
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9
Cell-Free Synthesis of Selenoproteins in High Yield and Purity for Selective Protein Tagging
Autor: Iresha D. Herath, Yi Jiun Tan, Daniella Goldfarb, Thomas Huber, Adarshi P. Welegedara, Angeliki Giannoulis, Mithun C. Mahawaththa, Gottfried Otting, Ansis Maleckis, Ruchira Bandara
Publikováno v: Chembiochem : a European journal of chemical biology. 22(8)
The selenol group of selenocysteine is much more nucleophilic than the thiol group of cysteine. Selenocysteine residues in proteins thus offer reactive points for rapid post-translational modification. Herein, we show that selenoproteins can be expre
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::57fcfdd236e788e3a08dcaaaba137e5e
https://pubmed.ncbi.nlm.nih.gov/33319405
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