Zobrazeno 1 - 10
of 20
pro vyhledávání: '"Ireneusz Sobolewski"'
Autor:
Beata Łubkowska, Edyta Czajkowska, Aleksandra Stodolna, Michał Sroczyński, Agnieszka Zylicz-Stachula, Ireneusz Sobolewski, Piotr M. Skowron
Publikováno v:
Microbial Cell Factories, Vol 22, Iss 1, Pp 1-16 (2023)
Abstract Background In spite of the fact that recombinant enzymes are preferably biotechnologically obtained using recombinant clones, the purification of proteins from native microorganisms, including those encoded by bacteriophages, continues. The
Externí odkaz:
https://doaj.org/article/1417d945ac91406f8d76dcbd4f672d87
Autor:
Beata Łubkowska, Edyta Czajkowska, Ireneusz Sobolewski, Natalia Krawczun, Agnieszka Żylicz-Stachula, Piotr M. Skowron
Publikováno v:
Microorganisms, Vol 11, Iss 9, p 2340 (2023)
Purification of bacteriophage-expressed proteins poses methodological difficulties associated with the need to process entire culture medium volume upon bacteriophage-induced bacterial cell lysis. We have used novel capsule glycosylase-depolymerase (
Externí odkaz:
https://doaj.org/article/7d2d0b022d7b496590a44d5deec7547e
Autor:
Beata Łubkowska, Edyta Czajkowska, Aleksandra Stodolna, Michał Sroczyński, Agnieszka Zylicz-Stachula, Ireneusz Sobolewski, Piotr M. Skowron
Publikováno v:
Microbial Cell Factories, Vol 22, Iss 1, Pp 1-1 (2023)
Externí odkaz:
https://doaj.org/article/15516527ee1e43eaa4e763c657803eb7
Autor:
Natalia Krawczun, Marta Bielawa, Kasjan Szemiako, Beata Łubkowska, Ireneusz Sobolewski, Agnieszka Zylicz-Stachula, Piotr M. Skowron
Publikováno v:
Microbial Cell Factories, Vol 19, Iss 1, Pp 1-10 (2020)
Abstract Background The biotechnology production of enzymes is often troubled by the toxicity of the recombinant products of cloned and expressed genes, which interferes with the recombinant hosts’ metabolism. Various approaches have been taken to
Externí odkaz:
https://doaj.org/article/25bdce40f8d64dcdb0ff9faaf790ae2f
Autor:
Ireneusz Sobolewski, Katarzyna Adamowicz, Anna Struck, Agnieszka Zylicz-Stachula, Piotr M. Skowron
Publikováno v:
Microorganisms, Vol 10, Iss 9, p 1782 (2022)
DNA purification methods are indispensable tools of molecular biology, used for many decades. Nevertheless, for certain specialized applications, the currently employed techniques are not sufficiently effective. While examining a number of the existi
Externí odkaz:
https://doaj.org/article/a6ec222654814a69982022e458009398
Autor:
Natalia Krawczun, Marta Bielawa, Kasjan Szemiako, Beata Łubkowska, Ireneusz Sobolewski, Agnieszka Zylicz-Stachula, Piotr M. Skowron
Publikováno v:
MethodsX, Vol 8, Iss , Pp 101340- (2021)
Genes encoding proteins ‘toxic' to recombinant host are difficult for cloning/expression and recombinant clones are unstable. Even tightly controlled inducible T7-lac, PBAD, PL, PR promoters are not totally silent in an uninduced state and thus not
Externí odkaz:
https://doaj.org/article/da6767defb184ce58655c7efe02c9cdb
Publikováno v:
Microorganisms, Vol 9, Iss 7, p 1522 (2021)
Bacteriophages of thermophiles are of increasing interest owing to their important roles in many biogeochemical, ecological processes and in biotechnology applications, including emerging bionanotechnology. However, due to lack of in-depth investigat
Externí odkaz:
https://doaj.org/article/37f181cfebe840f99e657b5f8fb78491
Autor:
Łubkowska Beata, Czajkowska Edyta, Stodolna Aleksandra, Sroczyński Michał, Zylicz-Stachula Agnieszka, Ireneusz Sobolewski, Skowron M. Piotr
Publikováno v:
Microbial Cell Factories. 22
Background In spite of the fact that recombinant enzymes are preferably biotechnologically obtained using recombinant clones, the purification of proteins from native microorganisms, including those encoded by bacteriophages, continues. The native ba
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::162661ef9b1ba3aa2c4d95e078df17a1
https://doi.org/10.1186/s12934-023-02086-2
https://doi.org/10.1186/s12934-023-02086-2
Publikováno v:
Microorganisms
Bacteriophages of thermophiles are of increasing interest owing to their important roles in many biogeochemical, ecological processes and in biotechnology applications, including emerging bionanotechnology. However, due to lack of in-depth investigat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::6341f5a530068cbb63c21a027bf0eb4d
https://pubmed.ncbi.nlm.nih.gov/34361957
https://pubmed.ncbi.nlm.nih.gov/34361957
Publikováno v:
Acta biochimica Polonica. 68(3)
The time of COVID-19 pandemic focused the attention of scientist to recognise the complex medical symptoms of the disease, modes of infection and possible therapies. The organisms’ response towards SARS-CoV-2 infection depends on many individual fa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::04dcdcd239ba89f1e9bb192a94c49793
https://pubmed.ncbi.nlm.nih.gov/34432408
https://pubmed.ncbi.nlm.nih.gov/34432408