Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Irene Beusch"'
Autor:
Ahmed Moursy, Antoine Cléry, Stefan Gerhardy, Katharina M. Betz, Sanjana Rao, Jarosław Mazur, Sébastien Campagne, Irene Beusch, Malgorzata M. Duszczyk, Mark D. Robinson, Vikram Govind Panse, Frédéric H.-T. Allain
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-15 (2023)
Abstract The conserved SR-like protein Npl3 promotes splicing of diverse pre-mRNAs. However, the RNA sequence(s) recognized by the RNA Recognition Motifs (RRM1 & RRM2) of Npl3 during the splicing reaction remain elusive. Here, we developed a split-iC
Externí odkaz:
https://doaj.org/article/4a88fb1ca6af451d9d41da0376069163
Publikováno v:
eLife, Vol 6 (2017)
HnRNP A1 regulates many alternative splicing events by the recognition of splicing silencer elements. Here, we provide the solution structures of its two RNA recognition motifs (RRMs) in complex with short RNA. In addition, we show by NMR that both R
Externí odkaz:
https://doaj.org/article/0fb8184bbd0d4ce6a03290896d1421a0
Targeted high throughput mutagenesis of the human spliceosome reveals itsin vivooperating principles
Autor:
Irene Beusch, Beiduo Rao, Michael Studer, Tetiana Luhovska, Viktorija Šukytė, Susan Lei, Juan Oses-Prieto, Em SeGraves, Alma Burlingame, Stefanie Jonas, Hiten D. Madhani
SUMMARYThe spliceosome is a staggeringly complex machine comprising, in humans, 5 snRNAs and >150 proteins. We scaled haploid CRISPR-Cas9 base editing to target the entire human spliceosome and interrogated the mutants using the U2 snRNP/SF3b inhibit
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c8b20b5802d9a3a49a44e4fd6e113c4e
https://doi.org/10.1101/2022.11.13.516350
https://doi.org/10.1101/2022.11.13.516350
Autor:
Ahmed Moursy, Antoine CLERY, Stefan Gerhardy, Katharina M. Betz, Sanjana Rao, Sébastien Campagne, Irene Beusch, Malgorzata Duszczyk, Mark Robinson, Vikram Panse, Frederic Allain
The conserved SR-like protein Npl3 promotes splicing of diverse pre-mRNAs. However, the RNA sequence(s) recognized by the RNA Recognition Motifs (RRM1 & RRM2) of Npl3 during the splicing reaction remain elusive. Here, we developed a split-iCRAC appro
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::91710a14b733a32f5c2387fa2bd851a2
https://doi.org/10.21203/rs.3.rs-2017343/v1
https://doi.org/10.21203/rs.3.rs-2017343/v1
Autor:
Ahmed Moursy, Antoine Cléry, Stefan Gerhardy, Katharina M. Betz, Sanjana Rao, Sébastien Campagne, Irene Beusch, Malgorzata M. Duszczyk, Mark D. Robinson, Vikram Govind Panse, Frédéric H.-T. Allain
The conserved SR-like protein Npl3 promotes splicing of diverse pre-mRNAs. However, the RNA sequence(s) recognized by the RNA Recognition Motifs (RRM1 & RRM2) of Npl3 during the splicing reaction remain elusive. Here, we developed a split-iCRAC appro
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ccc031de4878e7bffaffc752668087a8
https://doi.org/10.1101/2022.05.24.493251
https://doi.org/10.1101/2022.05.24.493251
Autor:
Sapna Ravindranathan, Christophe Maris, Frédéric H.-T. Allain, Irene Beusch, Fred F. Damberger, Sandrine Jayne, Georg Dorn
Publikováno v:
Nucleic Acids Research
Nucleic Acids Research, 48 (8)
Nucleic Acids Research, 48 (8)
The polypyrimidine tract binding protein (PTB) is a multi-domain protein involved in alternative splicing, mRNA localization, stabilization, polyadenylation and translation initiation from internal ribosome entry sites (IRES). In this latter process,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4435f9b029bcd3ab160b85236f36781a
https://pubmed.ncbi.nlm.nih.gov/32170319
https://pubmed.ncbi.nlm.nih.gov/32170319
Autor:
Beiduo Rao, Scott William Roy, Jade Sales-Lee, Daniela S. Perry, John R. Yates, Irene Beusch, Jolene K. Diedrich, Hiten D. Madhani, Bradley A. Bowser
Publikováno v:
Curr Biol
SUMMARYWe determined that over 40 spliceosomal proteins are conserved between many fungal species and humans but were lost during the evolution of S. cerevisiae, an intron-poor yeast with unusually rigid splicing signals. We analyzed null mutations i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c61563bbf824602886369630fb7f561c
https://doi.org/10.1016/j.cub.2021.09.004
https://doi.org/10.1016/j.cub.2021.09.004
Publikováno v:
eLife, Vol 6 (2017)
eLife, 6
eLife
eLife, 6
eLife
HnRNP A1 regulates many alternative splicing events by the recognition of splicing silencer elements. Here, we provide the solution structures of its two RNA recognition motifs (RRMs) in complex with short RNA. In addition, we show by NMR that both R
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::00742c808e2b1f21d8549949b0daae92
https://elifesciences.org/articles/25736
https://elifesciences.org/articles/25736