Zobrazeno 1 - 10
of 35
pro vyhledávání: '"Irena Bronstein"'
Publikováno v:
BioTechniques, Vol 21, Iss 3, Pp 520-524 (1996)
A unique combined luminescence assay for firefly (Photinus pyralis) luciferase and β-galactosidase (β-gal) reporter gene products is described. Luciferase and β-gal activities are determined with the same aliquot of cell lysate prepared from cells
Externí odkaz:
https://doaj.org/article/bfc5ce0297d9473fbdc4fb4ce0d55938
Publikováno v:
Biophysical journal. 109(7)
Internal organization and dynamics of the eukaryotic nucleus have been at the front of biophysical research in recent years. It is believed that both dynamics and location of chromatin segments are crucial for genetic regulation. Here we study the re
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::da537a1714ddc18ee6eccd50253d95a0
https://pubmed.ncbi.nlm.nih.gov/26445446
https://pubmed.ncbi.nlm.nih.gov/26445446
Publikováno v:
Journal of the American Chemical Society. 121:958-961
The excitation step of the intramolecular CIEEL generation in the triggered cleavage of spiroadamantyl-substituted dioxetane has been studied. The electron back-transfer (BET) process versus the direct chemiexcitation of the phenolate-anion emitter h
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::67efa84c0edffdf5b10700d07c3ee07d
https://doi.org/10.1021/ja982999k
https://doi.org/10.1021/ja982999k
Publikováno v:
The Journal of Physical Chemistry A. 102:5406-5414
A comparative spectral study on the intramolecular chemically initiated electron exchange luminescence (CIEEL) of five spiroadamantyl-substituted dioxetanes by various enzymatic and chemical triggers (alkaline phosphatase, HO-, and F-) in protic (H2O
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5cca4bde850873df1e4a45b14c9e7d46
https://doi.org/10.1021/jp973175c
https://doi.org/10.1021/jp973175c
Publikováno v:
SEIBUTSU BUTSURI KAGAKU. 42:265-279
The use of 1, 2-dioxetane chemiluminescent enzyme substrates, including AMPPD®, CSPD®, CDP® and CDP-Star® for alkaline phosphatase and Galacton-Star® substrate for β-galactosidase, provides highly sensitive detection for numerous immunoassay an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::14ffbc8a6528d1f1235d337f44e1b705
https://doi.org/10.2198/sbk.42.265
https://doi.org/10.2198/sbk.42.265
Publikováno v:
BioTechniques. 21:520-524
A unique combined luminescence assay for firefly (Photinus pyralis) luciferase and β-galactosidase (β-gal) reporter gene products is described. Luciferase and β-gal activities are determined with the same aliquot of cell lysate prepared from cells
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c2d9e29d29ccf3f0125ed5815fc026ab
https://doi.org/10.2144/96213pf01
https://doi.org/10.2144/96213pf01
Autor:
R. F. Vasil'ev, Friedemann W. Schneider, Waldemar Adam, Alexei V. Trofimov, Thomas Engel, Irena Bronstein, Brooks Edwards, Dirk Reinhardt
Publikováno v:
Journal of the American Chemical Society. 118:10400-10407
Intramolecular chemically initiated electron exchange luminescence (CIEEL) was studied in the alkaline phosphatase triggering of the spiroadamantyl-substituted 1,2-dioxetanes AMPPD and CSPD, which are widely employed in modern chemiluminescent bioass
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::bf1ea5b2704a09ad9a319503f5849284
https://doi.org/10.1021/ja961904g
https://doi.org/10.1021/ja961904g
Autor:
Chris S. Martin, Irena Bronstein
Publikováno v:
Journal of Bioluminescence and Chemiluminescence. 9:145-153
We investigated imaging of chemiluminescent signals from 1,2-dioxetanes with cooled CCD cameras. Non-radioactive detection methods for biomolecules utilizing these chemiluminescent substrates for alkaline phosphatase have been developed. Applications
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f05d9fc2b82be277b26f6ce72a04e90d
https://doi.org/10.1002/bio.1170090308
https://doi.org/10.1002/bio.1170090308
Autor:
Owen J. Murphy, Richard L. Cate, Irena Bronstein, Mark Wysk, Richard Tizard, John C. Voyta, Christian W. Ehrenfels
Publikováno v:
Genetic Analysis: Biomolecular Engineering. 8:102-106
We have used a chemiluminescent detection method to improve both the sensitivity and the speed of detection of human genes with oligonucleotide probes. A direct chemiluminescent substrate (AMPPD) was used in combination with an alkaline-phosphatase-l
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4c875272c88edb642ad55268414e38b5
https://doi.org/10.1016/1050-3862(91)90044-r
https://doi.org/10.1016/1050-3862(91)90044-r
Publikováno v:
Journal of Bioluminescence and Chemiluminescence. 5:1-4
With the advent of enzymatically induced chemiluminescence and improved instrumentation for luminometry, ultrasensitive detection of a wide variety of analytes is now possible using standard immunoassay and DNA probe formats. Model molecular orbital
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2818bae7a0bab316202761556402e3bc
https://doi.org/10.1002/bio.1170050102
https://doi.org/10.1002/bio.1170050102