Zobrazeno 1 - 10
of 35
pro vyhledávání: '"Irène Benveniste"'
Autor:
Sylvie Kandel, Danièle Werck-Reichhart, Franck Pinot, Irène Benveniste, Elizabeth Blée, Marc Morant
Publikováno v:
Journal of Biological Chemistry. 280:35881-35889
We cloned and characterized CYP709C1, a new plant cytochrome P450 belonging to the P450 family, that so far has no identified function except for clustering with a fatty acid metabolizing clade of P450 enzymes. We showed here that CYP709C1 is capable
Publikováno v:
Archives of Biochemistry and Biophysics. 391:180-187
Fatty acid omega-hydroxylation is involved in the biosynthesis of the plant cuticle, formation of plant defense signaling molecules, and possibly in the rapid catabolism of free fatty acids liberated under stress conditions. CYP94A2 is a cytochrome P
Autor:
Franck Pinot, Jean-Pierre Salaün, Renaud Le Bouquin, Irène Benveniste, Rachel A. Kahn, Francis Durst, M. Skrabs, Lukas Schreiber
Publikováno v:
European Journal of Biochemistry. 268:3083-3090
A full length cDNA encoding a new cytochrome P450-dependent fatty acid hydroxylase (CYP94A5) was isolated from a tobacco cDNA library. CYP94A5 was expressed in S. cerevisiae strain WAT11 containing a P450 reductase from Arabidopsis thaliana necessary
Autor:
Irène Benveniste, Lukas Schreiber, Jean-Pierre Salaün, Vincent Compagnon, M. Skrabs, Francis Durst, Franck Pinot
Publikováno v:
Biochemical Society Transactions. 28:867-870
The C(18) fatty acid derivatives 9,10-epoxystearic acid and 9,10-dihydroxystearic acid were hydroxylated on the terminal methyl by microsomes of yeast expressing CYP94A1 cloned from Vicia sativa. The reactions did not occur in incubations of microsom
Autor:
Francis Durst, Olivier Loreau, Jean-Pierre Noël, Lukas Schreiber, Irène Benveniste, Franck Pinot, Jean-Pierre Salaün
Publikováno v:
Biochemical Journal. 342:27-32
The major C18 cutin monomers are 18-hydroxy-9,10-epoxystearic and 9,10,18-trihydroxystearic acids. These compounds are also known messengers in plant-pathogen interactions. We have previously shown that their common precursor 9,10-epoxystearic acid w
Publikováno v:
Biochemical and Biophysical Research Communications. 261:156-162
A full length cDNA encoding a new cytochrome P450-dependent fatty acid hydroxylase (CYP94A2) was isolated from a Vicia sativa library. CYP94A2 displays 58% sequence identity with CYP94A1, a fatty acid omega-hydroxylase isolated from the same material
Autor:
Fadi Adas, Gabriel Philipps, Nathalie Tijet, Francis Durst, Jean-Pierre Salaün, Irène Benveniste
Publikováno v:
Biochemical and Biophysical Research Communications. 243:688-693
The A. thaliana EST database was screened using consensus motifs derived from P450 families CYP52 and CYP4 catalyzing the omega-hydroxylation of fatty acids and alkanes in Candida and in mammals. One EST cDNA fragment was detected in this way and the
Publikováno v:
Phytochemistry. 42:927-933
A microsomal preparation isolated from first leaves of 25-day-old spinach catalysed the hydroxylation of ecdysone to produce the insect moulting hormone, 20-hydroxyecdysone. Hydroxylation was dependent on NADPH and molecular oxygen, and was inhibited
Publikováno v:
Biochemical Society Transactions. 20:353-357
The spontaneous reaction of organic molecules with atmospheric oxygen is a spin-forbidden, and therefore slow. process. In aerobic organisms a few proteins, bearing a prosthetic metal or flavin to overcome this spin barrier, catalyse this crucial int
Publikováno v:
Analytical Biochemistry. 197:125-131
The potentiality of the Triton X-114 phase separation technique for the purification of proteins from plant microsomal membranes has been investigated. It was shown that glycerol significantly lowers the cloud point of Triton X-114 solutions in water