Zobrazeno 1 - 10
of 15
pro vyhledávání: '"Ipek Tasan"'
Autor:
Qin Peng, Ziliang Huang, Kun Sun, Yahan Liu, Chi Woo Yoon, Reed E. S. Harrison, Danielle L. Schmitt, Linshan Zhu, Yiqian Wu, Ipek Tasan, Huimin Zhao, Jin Zhang, Sheng Zhong, Shu Chien, Yingxiao Wang
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-15 (2022)
Imaging non-repetitive loci in living cells remains challenging. Here, the authors engineered an inducible system whereby biomolecular assemblies can be guided to specific genomic loci by a nuclease-defective Cas9, allowing the simultaneous imaging a
Externí odkaz:
https://doaj.org/article/ce8a2c245cbe4bf2851e171ec04346fc
Autor:
Manasi Lingamaneni, Guanhua Xun, Ipek Tasan, Che Yang, Huimin Zhao, Teresa A. Martin, Shireen Abesteh, Surbhi Jain, Sherri Ho
Publikováno v:
ACS Synthetic Biology. 10:1320-1327
CRISPR/Cas9 is a powerful genome editing tool, but its off-target cleavage activity can result in unintended adverse outcomes for therapeutic applications. Here we report the design of a simple tunable CRISPR controller in which a chemically inducibl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::86cae2e02df21150a7cfd3b968a9e604
https://doi.org/10.1021/acssynbio.0c00548
https://doi.org/10.1021/acssynbio.0c00548
Autor:
David M. Gilbert, Shin-ichiro Hiraga, Toyoaki Natsume, Danny Leung, Ipek Tasan, Xuemeng Zhou, Anne D. Donaldson, Meng Zhang, Daniel A. Bartlett, Xiaowen Lyu, Takayo Sasaki, Timour Baslan, Amar M. Singh, Victor G. Corces, Masato T. Kanemaki, Lotte P. Watts, Kyle N. Klein, Stephen Dalton, Huimin Zhao, Peiyao A. Zhao
Publikováno v:
Science
Replication timing organizes epigenome The temporal order of DNA replication is conserved from yeast to humans, but its biological significance remains unclear. Klein et al. eliminated the protein RIF1, a master regulator of replication timing, in se
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::90b4e2880380bab43282277f04c7f32f
https://doi.org/10.1126/science.aba5545
https://doi.org/10.1126/science.aba5545
Publikováno v:
ACS Synthetic Biology. 9:2502-2514
Tools for live cell imaging of multiple nonrepetitive genomic loci in mammalian cells are necessary to study chromatin dynamics. Here, we report a new system based on two chromosomally integrated orthogonal irregular repeat arrays and particularly a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b1d4ffc1a79f3a2e6ffe98dc746e46e0
https://doi.org/10.1021/acssynbio.0c00295
https://doi.org/10.1021/acssynbio.0c00295
Autor:
Yuanqing Lan, Meng Zhang, Qiqi Tian, Surbhi Jain, Ipek Tasan, Yi Yu, Huimin Zhao, Hugh Yeh, Yijun Guo
Publikováno v:
Nature Chemical Biology. 16:387-390
Here, we report a rapid CRISPR–Cas9-mediated gene knock-in strategy that uses Cas9 ribonucleoprotein and 5′-modified double-stranded DNA donors with 50-base-pair homology arms and achieved unprecedented 65/40% knock-in rates for 0.7/2.5 kilobase
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b56911a6f2842f8d3efcdccf8f41b031
https://doi.org/10.1038/s41589-019-0432-1
https://doi.org/10.1038/s41589-019-0432-1
Publikováno v:
ACS synthetic biology. 10(3)
Inserting custom designed DNA sequences into the mammalian genome plays an essential role in synthetic biology. In particular, the ability to introduce foreign DNA in a site-specific manner offers numerous advantages over random DNA integration. In t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::bbb0b3390a5da92703ca017b6edc4e24
https://pubmed.ncbi.nlm.nih.gov/33596056
https://pubmed.ncbi.nlm.nih.gov/33596056
Autor:
Peiyao A. Zhao, Masato T. Kanemaki, Lotte P. Watts, Toyoaki Natsume, Daniel A. Bartlett, Xuemeng Zhou, Anne D. Donaldson, Ipek Tasan, Amar M. Singh, Huimin Zhao, Shin-ichiro Hiraga, Victor G. Corces, Kyle N. Klein, Xiaowen Lyu, Danny Leung, Stephen Dalton, David M. Gilbert
DNA is replicated in a defined temporal order termed the replication timing (RT) program. RT is spatially segregated in the nucleus with early/late replication corresponding to Hi-C A/B chromatin compartments, respectively. Early replication is also
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::883b525b681ac225d8b031371435b76b
https://doi.org/10.1101/2019.12.28.890020
https://doi.org/10.1101/2019.12.28.890020
Autor:
Pu Xue, Han Xiao, Ipek Tasan, Ran Chao, Mohammad HamediRad, Zehua Bao, Jing Liang, Huimin Zhao
Publikováno v:
Nature Biotechnology. 36:505-508
We developed a CRISPR-Cas9- and homology-directed-repair-assisted genome-scale engineering method named CHAnGE that can rapidly output tens of thousands of specific genetic variants in yeast. More than 98% of target sequences were efficiently edited
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::49875b60b4406f53d51c4c72ec81a87d
https://doi.org/10.1038/nbt.4132
https://doi.org/10.1038/nbt.4132
Autor:
Yi, Yu, Yijun, Guo, Qiqi, Tian, Yuanqing, Lan, Hugh, Yeh, Meng, Zhang, Ipek, Tasan, Surbhi, Jain, Huimin, Zhao
Publikováno v:
Nature chemical biology. 16(4)
Here, we report a rapid CRISPR-Cas9-mediated gene knock-in strategy that uses Cas9 ribonucleoprotein and 5'-modified double-stranded DNA donors with 50-base-pair homology arms and achieved unprecedented 65/40% knock-in rates for 0.7/2.5 kilobase inse
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::f25cdd9f218785cbf4e53a8f5f62003f
https://pubmed.ncbi.nlm.nih.gov/31942048
https://pubmed.ncbi.nlm.nih.gov/31942048
Autor:
Huimin Zhao, Ipek Tasan
Publikováno v:
ACS synthetic biology. 6(9)
CRISPR/Cas9 system has accelerated research across many fields since its demonstration for genome editing. CRISPR also offers vast therapeutic potential, but an important hurdle of this technology is the off-target mutations it can induce. In this vi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::60c9bbeabbadabde66bfd63e89f7641f
https://pubmed.ncbi.nlm.nih.gov/28911233
https://pubmed.ncbi.nlm.nih.gov/28911233