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Additional file 4: of Expression of ID4 protein in breast cancer cells induces reprogramming of tumour-associated macrophages

Autor: Donzelli, Sara, Milano, Elisa, Pruszko, Magdalena, Sacconi, Andrea, Masciarelli, Silvia, Iosue, Ilaria, Melucci, Elisa, Gallo, Enzo, Terrenato, Irene, Mottolese, Marcella, Zylicz, Maciej, Zylicz, Alicja, Fazi, Francesco, Blandino, Giovanni, Fontemaggi, Giulia

Table S2 Predictive power of ID4, CD68 and the macrophage signature (MacSig) comprising eight widely used markers (CD14, CD105, CD11b, CD68, CD93, CD33, IL4R, CD163) for the mononuclear phagocyte system [37]. Analysis was performed using datasets dep

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Additional file 5: of Expression of ID4 protein in breast cancer cells induces reprogramming of tumour-associated macrophages

Table S3 mRNAs modulated in an ID4-dependent manner in differentiated HL60 cells cultured with conditioned medium from control (CM EV) or ID4-overexpressing (CM ID4) MDA-MB-468 cells. The presence of HIF-1 consensus sequences on promoters was evaluat

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Additional file 9: of Expression of ID4 protein in breast cancer cells induces reprogramming of tumour-associated macrophages

Figure S6 Differentiated U937 cells transfected with an empty vector (EV) or a granulin (GRN) expression vector and subsequently cultivated in the presence of CM from MDA-MB-468 cells were evaluated for their differentiation state (percentage of CD11

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Additional file 6: of Expression of ID4 protein in breast cancer cells induces reprogramming of tumour-associated macrophages

Figure S2. Predictive power of ID4 mRNA expression for overall survival (OS) was evaluated by Kaplan-Meier analysis on the TCGA cohort in BLBCs showing high or low CD68 (a and b) or macrophage signature (MacSig) (c and d) levels. Macrophage signature

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Additional file 1: of Expression of ID4 protein in breast cancer cells induces reprogramming of tumour-associated macrophages

Figure S3 Growth curve of MDA-MB-468 cells depleted (si-ID4) or not (si-SCR) of ID4 expression by siRNA transfection (a). Cells were transfected for 16Â hours, and then equal numbers of cells were plated and counted at the indicated time points. Eff

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Additional file 3: of Expression of ID4 protein in breast cancer cells induces reprogramming of tumour-associated macrophages

Figure S1. Comparison of ID4 mRNA expression in basal-like breast cancer (BLBC) and triple-negative breast cancer (TNBC) versus all other breast cancer subtypes (Others) in the indicated representative datasets [19, 22, 60]. (PDF 143 kb)

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Additional file 7: of Expression of ID4 protein in breast cancer cells induces reprogramming of tumour-associated macrophages

Figure S4 a Modulation of selected genes modulated in the TLDA was validated by RT-qPCR in differentiated HL60 cells cultured in CM from ID4-overexpressing (CM ID4-HA) or control (CM EV) MDA-MB-468 cells (left panel). The same transcripts were analys

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Additional file 8: of Expression of ID4 protein in breast cancer cells induces reprogramming of tumour-associated macrophages

Figure S5 a Expression of miR-107, miR-15b and miR-195 in differentiated HL60 cells cultured with CM from control (CM EV) or ID4-overexpressing (CM ID4) MDA-MB-468 cells. bâ e Expression of miR-15b and miR-195 in HL60 and U937 cells cultured with CM

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