CHOGlycoNET: Comprehensive glycosylation reaction network for CHO cells

Autor: Pavlos Kotidis, Roberto Donini, Johnny Arnsdorf, Anders Holmgaard Hansen, Bjørn Gunnar Rude Voldborg, Austin W.T. Chiang, Stuart M. Haslam, Michael Betenbaugh, Ioscani Jimenez del Val, Nathan E. Lewis, Frederick Krambeck, Cleo Kontoravdi

Publikováno v: Kotidis, P, Donini, R, Arnsdorf, J, Hansen, A H, Voldborg, B G R, Chiang, A W T, Haslam, S M, Betenbaugh, M, Jimenez del Val, I, Lewis, N E, Krambeck, F & Kontoravdi, C 2022, ' CHOGlycoNET: Comprehensive glycosylation reaction network for CHO cells ', Metabolic Engineering, vol. 76, pp. 87-96 . https://doi.org/10.1016/j.ymben.2022.12.009

Chinese hamster ovary (CHO) cells are extensively used for the production of glycoprotein therapeutics proteins, for which N-linked glycans are a critical quality attribute due to their influence on activity and immunogenicity. Manipulation of protei

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e9c4ce3ae7c163ab17f6d45976286d5d
https://orbit.dtu.dk/en/publications/e3b57c88-ddef-4b08-a8b0-0490f1e1b7ba

Rapid Antibody Glycoengineering in CHO Cells Via RNA Interference and CGE-LIF N-Glycomics

Autor: Pavlos Kotidis, Karen M. Polizzi, Ioscani Jimenez del Val, Cleo Kontoravdi, Stuart M. Haslam, Itzcóatl A Gómez, Masue Marbiah, Roberto Donini

Publikováno v: Glycosylation ISBN: 9781071616840

The impact of the glycan distribution on the in vivo function and half-life of monoclonal antibodies has long motivated the genetic engineering of producer cells to achieve structures that enhance efficacy, safety and stability. To facilitate glycoen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::f849a58cc56ce81834fc0ba029cf599e
https://doi.org/10.1007/978-1-0716-1685-7_7

An integrated modular framework for modeling the effect of ammonium on the sialylation process of monoclonal antibodies produced by CHO cells

Autor: Nathan E Lewis, Iman Shahidi Pour Savizi, Ehsan Motamedian, Seyed Abbas Shojaosadati, Ioscani Jimenez del Val

Publikováno v: Biotechnology journalREFERENCES. 16(8)

Monoclonal antibodies (mABs) have emerged as one of the most important therapeutic recombinant proteins in the pharmaceutical industry. Their immunogenicity and therapeutic efficacy are influenced by post-translational modifications, specifically the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b2e0654d50dfb0a6d7e2522f782ce45b
https://pubmed.ncbi.nlm.nih.gov/34021707

Model-based operational optimisation of a microbial bioprocess converting terephthalic acid to biomass

Autor: Kevin E. O’Connor, Niall Beagan, Ioscani Jimenez del Val

Publikováno v: Biochemical Engineering Journal. 158:107576

Polyethylene terephthalate (PET) is a major contributor to plastic pollution due to its widespread presence in synthetic fibres. As an intermediate in the chemical recycling of PET, disodium terephthalate (Na2TA) can be harnessed as a cheap microbial

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::484b6c0681f5adec266b3fc5391c42aa
https://doi.org/10.1016/j.bej.2020.107576

A multi-pronged investigation into the effect of glucose starvation and culture duration on fed-batch CHO cell culture

Autor: Dietmar Weilguny, Christian Müller, Anne Mathilde Lund, Jette Wagtberg Sen, Cleo Kontoravdi, Søren Kofoed Rasmussen, Yuzhou Fan, Ioscani Jimenez del Val, Michael J. Betenbaugh, Mikael Rørdam Andersen, Deniz Baycin-Hizal

Publikováno v: Biotechnology and Bioengineering. 112:2172-2184

In this study, omics-based analysis tools were used to explore the effect of glucose starvation and culture duration on monoclonal antibody (mAb) production in fed-batch CHO cell culture to gain better insight into how these parameters can be control

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::cf3ff5f631ee5d6f7a7ba698974c7826
https://doi.org/10.1002/bit.25620