Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Ioanna P. Petrounia"'
Autor:
John G. Geisler, Yin Liang, Robyn Williams, Gary W. Caldwell, Keith T. Demarest, Margery A. Connelly, Eugene B. Grant, Matthew J. Todd, Ioanna P. Petrounia, Brett P. Monia, Hossein Askari, Sanjay Bhanot, Jenson Qi, Geoffrey T. Struble, Micheal D. Gaul, Wensheng Lang, Charles Smith, Shariff Bayoumy, Selyna Mai, Ping Wang
Publikováno v:
Journal of Lipid Research, Vol 53, Iss 6, Pp 1106-1116 (2012)
Diacylglycerol acyltransferase (DGAT) catalyzes the final step in triglyceride (TG) synthesis. There are two isoforms, DGAT1 and DGAT2, with distinct protein sequences and potentially different physiological functions. To date, the ability to determi
Autor:
Ehab Khalil, Anna C. Maroney, Kevin J. Moriarty, Farah Ali, Robert A. Galemmo, Dana L. Johnson, Carl S. Crysler, Holly K. Koblish, Ioanna P. Petrounia, Thomas Garrabrant, Jahanvi Maisuria
Publikováno v:
Bioorganic & Medicinal Chemistry Letters. 16:5778-5783
A new class of Aurora-A inhibitors have been identified based on the 2-amino-pyrrolo[2,3-d]pyrimidine scaffold. Here, we describe the synthesis and SAR of this novel series. We report compounds which exhibit nanomolar activity in the Aurora-A biochem
Autor:
Ioanna P. Petrounia, Frances H. Arnold
Publikováno v:
Current Opinion in Biotechnology. 11:325-330
By providing a simple and reliable route to enzyme improvement, directed evolution has emerged as a key technology for enzyme engineering and biocatalysis. Recent advances include the evolution of a novel catalytic activity using the alpha/beta barre
Autor:
Ioanna P. Petrounia, Ralph M. Pollack
Publikováno v:
Biochemistry. 37:700-705
The nature of hydrogen bonding to the intermediate of the reaction catalyzed by 3-oxo-Delta5-steroid isomerase (KSI) was investigated. Substituted phenols bind tightly to the active site of the D38N mutant of KSI, and are analogs of the intermediate
Autor:
Celine Schalk-Hihi, Mark R. Player, Carsten Schubert, Raymond J. Patch, Benjamin Brandt, Hong-Chang Ma, Barry A. Springer, Ioanna P. Petrounia, Ingrid C. Deckman, Geoffrey T. Struble, John C. Spurlino
Publikováno v:
The Journal of biological chemistry. 282(6)
The cFMS proto-oncogene encodes for the colony-stimulating factor-1 receptor, a receptor-tyrosine kinase responsible for the differentiation and maturation of certain macrophages. Upon binding its ligand colony-stimulating factor-1 cFMS autophosphory
Publikováno v:
Chembiochem : a European journal of chemical biology. 3(8)
The selective oxidation of the 6-hydroxy group of D-glucose to introduce an aldehyde functionality is not catalyzed by known oxidase enzymes. Selective functionalization at the glucose C-6 position in oligoand polysaccharides is a synthetically usefu
Publikováno v:
Protein engineering. 14(9)
We have used directed evolution methods to express a fungal enzyme, galactose oxidase (GOase), in functional form in Escherichia coli. The evolved enzymes retain the activity and substrate specificity of the native fungal oxidase, but are more thermo
Publikováno v:
Biochemistry. 39(1)
3-Oxo-Delta(5)-steroid isomerase (KSI) catalyzes the isomerization of a variety of 3-oxo-Delta(5)-steroids to their conjugated Delta(4) isomers. The mechanism involves sequential enolization and ketonization, with Asp-38 acting to transfer a proton f