Zobrazeno 1 - 10
of 13
pro vyhledávání: '"Ioanna A. Gorbunova"'
Autor:
Ioanna A. Gorbunova, Maxim E. Sasin, Dmitry V. Yachkov, Denis A. Volkov, Alexei D. Vedyaykin, Andrey A. Nikiforov, Oleg S. Vasyutinskii
Publikováno v:
Biomolecules, Vol 13, Iss 2, p 256 (2023)
Thorough study of composition and fluorescence properties of a commercial reagent of active equine NAD-dependent alcohol dehydrogenase expressed and purified from E. coli has been carried out. Several experimental methods: spectral- and time-resolved
Externí odkaz:
https://doaj.org/article/4079d9d85d5c4d468aafb1fb9c1c809e
Autor:
Ioanna A. Gorbunova, Maxim E. Sasin, Anna A. Zhikhoreva, Andrey V. Belashov, Dina M. Beltukova, Irina V. Semenova, Oleg S. Vasyutinskii
Publikováno v:
Photonics, Vol 10, Iss 1, p 9 (2022)
The fluorescence anisotropy of photosensitizers Radachlorin and chlorin e6 was studied using the time-resolved single photon-counting technique under one- and two-photon excitation within the Soret absorption band. A very small negative anisotropy wa
Externí odkaz:
https://doaj.org/article/f562125a891543718f65faead4d074e8
Autor:
Oleg S. Vasyutinskii, Yaroslav M. Beltukov, Ioanna A. Gorbunova, Alexander A. Semenov, Andrey G. Smolin, M. E. Sasin, Dmitrii P. Golyshev
Publikováno v:
The Journal of Physical Chemistry B. 125:9692-9707
The dynamics of polarized fluorescence in NADH in alcohol dehydrogenase (ADH) in buffer solution has been studied using the TCSPC spectroscopy. A global fit procedure was used for determination of the fluorescence parameters from experiment. The inte
Autor:
M. E. Sasin, Ioanna A. Gorbunova, Alexander A. Semenov, Oleg S. Vasyutinskii, Yaroslav M. Beltukov
Publikováno v:
Physical Chemistry Chemical Physics. 22:18155-18168
We present the results of experimental and theoretical studies of fast anisotropic relaxation and rotational diffusion in the first electron excited state of biological coenzyme NADH in water-ethanol solutions. The experiments have been carried out b
Autor:
Ioanna A. Gorbunova, Marina K. Danilova, Maxim E. Sasin, Victor P. Belik, Dmitrii P. Golyshev, Oleg S. Vasyutinskii
Publikováno v:
SSRN Electronic Journal.
Autor:
M. E. Sasin, Andrey G. Smolin, Marina K. Krasnopevtceva, Dmitrii P. Golyshev, Viktor P. Belik, Oleg S. Vasyutinskii, Ioanna A. Gorbunova
Publikováno v:
Optics in Health Care and Biomedical Optics XI.
The paper presents the study of anisotropic fluorescence decay of FAD in water-methanol solutions of different concentrations in the range of 0-80%. The fluorescence decay parameters: decay times, corresponding weighting coefficients, fluorescence an
The role of microenvironment in the mechanisms of f luorescence decay in free and protein-bound NADH
Autor:
M. E. Sasin, Marina K. Krasnopevtceva, Yaroslav M. Beltukov, Andrey G. Smolin, Dmitry P. Golyshev, Ioanna A. Gorbunova, Oleg S. Vasyutinskii
Publikováno v:
Optics in Health Care and Biomedical Optics XI.
The influence of solution polarity and viscosity on fluorescence decay parameters in free and protein-bound NADH was studied. The fluorescence in NADH dissolved in water, methanol, ethanol, propylene glycol, and alcohol dehydrogenase-containing solut
Observation of Anisotropic Relaxation in Biological Molecules with Subpicosecond Temporal Resolution
Publikováno v:
Technical Physics Letters. 46:158-160
A new pump–probe technique has been developed for investigation of anisotropic relaxation and energy transfer processes in excited states of polyatomic molecules excited by femtosecond laser pulses. The proposed method was applied for studying the
Autor:
Andrey G. Smolin, Oleg S. Vasyutinskii, M. E. Sasin, Jesús Rubayo-Soneira, Ioanna A. Gorbunova
Publikováno v:
The journal of physical chemistry. B. 124(47)
The dynamics of polarized fluorescence in reduced nicotinamide adenine dinucleotide (NADH) at 460 nm under two-photon excitation at 720 nm by femtosecond laser pulses in water-methanol solutions has been studied experimentally and theoretically as a
Autor:
Ioanna A. Gorbunova, Jesús Rubayo-Soneira, Nikolai O. Bezverkhnii, Oleg S. Vasyutinskii, M. E. Sasin
Publikováno v:
2020 International Conference Laser Optics (ICLO).
We studied polarized fluorescence in biological coenzyme NADH in water-methanol solutions of various viscosity and polarity upon two-photon excitation with femtosecond laser pulses at 720 nm. Polarization anisotropy, fluorescence lifetimes, and rotat