Determination of Antibody Population Distributions for Virus-Antibody Conjugates by Charge Detection Mass Spectrometry

Autor: Ioana L. Aanei, Kevin M. Bond, Martin F. Jarrold, Matthew B. Francis

Publikováno v: Analytical Chemistry. 92:1285-1291

Virus-like particle (VLP) conjugates are being developed for biomedical applications; however, there is a lack of quantitative analytical methods to measure the extent of conjugation and modification of VLP based therapeutics. Charge detection mass s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::569e35cc8c86057577ecac3a8b1b5aab
https://doi.org/10.1021/acs.analchem.9b04457

Encapsulation of Negatively Charged Cargo in MS2 Viral Capsids

Autor: Ioana L, Aanei, Jeff E, Glasgow, Stacy L, Capehart, Matthew B, Francis

Publikováno v: Methods in molecular biology (Clifton, N.J.). 1776

Encapsulation into virus-like particles is an efficient way of loading cargo of interest for delivery applications. Here, we describe the encapsulation of proteins with tags comprising anionic amino acids or DNA and gold nanoparticles with negative s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::246cbc19eb4440653783d2c44d918849
https://pubmed.ncbi.nlm.nih.gov/29869251

Dual Surface Modification of Genome-Free MS2 Capsids for Delivery Applications

Autor: Matthew B. Francis, Ioana L. Aanei

Publikováno v: Methods in Molecular Biology ISBN: 9781493978069

One of the hallmarks of virus-like particles (VLPs) is the fact that they possess distinguishable interior and exterior surfaces. Taking advantage of our knowledge of the amino acid location from X-ray crystal structures, we have developed a series o

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::15648045ac943c2b9923923ec189a866
https://doi.org/10.1007/978-1-4939-7808-3_40

Synthetically Modified Viral Capsids as Versatile Carriers for Use in Antibody-Based Cell Targeting

Autor: Michelle E. Farkas, Adel M. ElSohly, Astraea Jager, Ioana L. Aanei, Garry P. Nolan, Sean C. Bendall, Matthew B. Francis, Chawita Netirojjanakul

Publikováno v: Bioconjugate chemistry, vol 26, iss 8

The present study describes an efficient and reliable method for the preparation of MS2 viral capsids that are synthetically modified with antibodies using a rapid oxidative coupling strategy. The overall protocol delivers conjugates in high yields a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e0d218ab0d4fc58fba8af1cc1438d031
https://doi.org/10.1021/acs.bioconjchem.5b00226

Stable Disk Assemblies of a Tobacco Mosaic Virus Mutant as Nanoscale Scaffolds for Applications in Drug Delivery

Autor: Emily C. Hartman, Daniel T. Finley, Ioana L. Aanei, Matthew B. Francis, Kenneth H. Downing, Kenneth Han, Max Fishman, Joel A. Finbloom, Michel T. Dedeo

Publikováno v: Bioconjugate chemistry, vol 27, iss 10
Finbloom, JA; Han, K; Aanei, IL; Hartman, EC; Finley, DT; Dedeo, MT; et al.(2016). Stable Disk Assemblies of a Tobacco Mosaic Virus Mutant as Nanoscale Scaffolds for Applications in Drug Delivery. Bioconjugate Chemistry, 27(10), 2480-2485. doi: 10.1021/acs.bioconjchem.6b00424. Lawrence Berkeley National Laboratory: Retrieved from: http://www.escholarship.org/uc/item/0nk9c1hm

© 2016 American Chemical Society. Current approaches to nanoscale therapeutic delivery rely on the attachment of a drug of interest to a nanomaterial scaffold that is capable of releasing the drug selectively in a tumor environment. One class of nan

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::93c335bd809a2b811711187f8776cf5c
https://escholarship.org/uc/item/0nk9c1hm