Recombinant Expression, Renaturation and Biological Activity of Temperature-Responsive Nanobodies against Aflatoxin B1

Autor: ZHANG Leping, TU Zhui, LI Yanping, LI Xiaojiang, SHUAI Wenyuan, ZHANG Hang, HE Qinghua

Publikováno v: Shipin Kexue, Vol 44, Iss 18, Pp 141-148 (2023)

In this study, temperature-responsive anti-aflatoxin B1 (AFB1) nanoantibodies were obtained by fusion expression of elastin-like polypeptides (ELPs) of different lengths with nanoantibodies. The recombinant expression vectors pET30a-G8-ELP20, pET30a-

Externí odkaz: https://doaj.org/article/15a64b4988834771b46b8d86e98b34b3

Engineered elastin-like polypeptides: An efficient platform for enhanced cancer treatment

Autor: Aiguo Jiang, Xinqiang Guan, Lianping He, Xingang Guan

Publikováno v: Frontiers in Pharmacology, Vol 13 (2023)

Drug delivery systems (DDSs) have recently gained widespread attention for improving drug loading and delivery efficiency in treating many cancers. Elastin-like polypeptides (ELPs) are synthetic peptides derived from a precursor of elastin (tropoelas

Externí odkaz: https://doaj.org/article/9d9c02b7db804f1aaae168546a94cee6

Cloning, expression and functional assessment of elastin like protein: a new protein in medical engineering

Autor: Neda Rezaeinasab, Mehdi Zeinoddini, AliReza Saeeidinia

Publikováno v: Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd, Vol 25, Iss 5, Pp 361-370 (2017)

Introduction: Elastin like protein or ELP is a synthetic biopolymer consisting the pentapeptide repeats of VPGXG (X can be any amino acid except Pro). This protein is the thermal responsive polypeptide that undergoes a reversible phase transition. At

Externí odkaz: https://doaj.org/article/74a58337c1ca4dd19a009df7c6cd8586

Production, purification and characterization of an elastin-like polypeptide containing the Ile-Lys-Val-Ala-Val (IKVAV) peptide for tissue engineering applications

Autor: Katell Bathany, Elsa Chevron, Sébastien Lecommandoux, Joëlle Amédée, Elisabeth Garanger, Mattia Pasqua, Bertrand Garbay, Zoeisha S. Chinoy, Hugo Oliveira, Bruno Paiva dos Santos, Christophe Cullin

Publikováno v: Journal of Biotechnology
Journal of Biotechnology, Elsevier, 2019, 298, pp.35-44. ⟨10.1016/j.jbiotec.2019.04.010⟩
Journal of Biotechnology, 2019, 298, pp.35-44
Journal of Biotechnology, 2019, 298, pp.35-44. ⟨10.1016/j.jbiotec.2019.04.010⟩

International audience; Elastin-like polypeptides (ELPs) are biocompatible-engineered polypeptides, with promising interest in tissue engineering due to their intrinsic biological and physical properties, and their ease of production. The IKVAV (Ile-

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0dcbf188ebb7c1cc182d586db19d84cf
https://hal.archives-ouvertes.fr/hal-02100654/document

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