Detection of monoclonal or oligoclonal integration of bovine leukemia virus proviral DNA by inverse polymerase chain reaction for diagnosis of enzootic bovine leucosis

Autor: Yoshiyasu Kobayashi, Kana Sakaguchi, Yusuke Tanaka, Masaki Maezawa, Hisashi Inokuma, Kenichi Watanabe

Publikováno v: Comparative Clinical Pathology. 30:711-714

Enzootic bovine leukosis (EBL) is characterized by systemic B-cell lymphoma associated with bovine leukemia virus (BLV) infection in cattle over 3 years old. This study evaluated the use of inverse polymerase chain reaction (PCR) as a diagnostic meth

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::c160170cd22c2ec3b226674b78edf91f
https://doi.org/10.1007/s00580-021-03265-6

Breakpoint characterization of a rare alpha 0 ‐thalassemia deletion using targeted locus amplification on genomic DNA

Autor: Cornelis L. Harteveld, Stefan J. White, Emile de Meijer, Henk P. J. Buermans, Quint P Hottentot

Publikováno v: International Journal of Laboratory Hematology, 43(6), 1628-1634. WILEY

Introduction The high-sequence homology of the α-globin-gene cluster is responsible for microhomology-mediated recombination events during meiosis, resulting in a high density of deletion breakpoints within a 10 kb region. Commonly used deletion det

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b8435c45162f811a3e902d97c1cb1c7f
https://doi.org/10.1111/ijlh.13651

Evaluation of CRISPR/Cas9 site-specific function and validation of sgRNA sequence by a Cas9/sgRNA-assisted reverse PCR technique

Autor: Song Zongming, Jiaojiao Wang, Yuanmeng Wei, Huang Zixu, Li Xiaoli, Jiamu Zhou, Miao Li, Beibei Zhang, Pingling Shi, He Tang, Wang Yange

Publikováno v: Analytical and Bioanalytical Chemistry

Graphical abstract The effective application of the clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system in biology, medicine and other fields is hindered by the off-target effects and loci-affinity of Cas9-sgRNA, especially

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::49fb3642433024aa5160f256f487bfd7
https://doi.org/10.1007/s00216-021-03173-2

UPAYA PENINGKATAN KUALITAS TANAMAN KEDELAI (Glycine max L. Merill) MELALUI PEMANFAATAN BIOTEKNOLOGI DALAM MENGATASI KELANGKAAN PANGAN

Autor: Muh Afif Juradi, Edi Tando

Publikováno v: AGROTEK: Jurnal Ilmiah Ilmu Pertanian. 3:113-128

Population growth the rapidly is currently a major challenges to business world food supply. The development of biotechnology begins with technology genetically engineered has accelerated . Products of biotechnology plants resembling plants provenanc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::818a96d676b2afa011f703e829627ff6
https://doi.org/10.33096/agrotek.v3i2.79

Application of Chromosome Conformation Capture Method for Detection MYC/TRD Chromosomal Translocation in Leukemia Cell Line

Autor: Gholamreza Tavoosidana, Mohammad Hossein Ghahremani, Mostafa Akbariqomi, Reza Heidari, Roya Karimi, Moloud Absalan, Shilan Shafei, Zahra Jabbarpour

Publikováno v: International Journal of Hematology-Oncology and Stem Cell Research
International Journal of Hematology-Oncology and Stem Cell Research, 2020, ⟨10.18502/ijhoscr.v14i3.3729⟩
International Journal of Hematology-Oncology and Stem Cell Research, Vol 14, Iss 3 (2020)

Background: Chromosomal breakpoints are the most common cause of hereditary diseases and cancers. Today, many standard clinical methods such as cytogenetic and PCR based techniques are used which have limitation regarding detection resolution. Chromo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::12ebed36bfecacc087c88b80be968777
https://hal.archives-ouvertes.fr/hal-02956978