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Simplified method for the collection, storage, and comet assay analysis of DNA damage in whole blood
Autor:
Mark D. Evans, George D. D. Jones, Kwok K. So, Hussein H. K. Abbas, Karen J. Bowman, Mahsa Karbaschi, Marcus S. Cooke, Roger W. L. Godschalk, Intisar Abdalla, Kamla Al-Salmani, Sjors Schulpen
Publikováno v:
Free Radical Biology and Medicine, 51(3), 719-725. Elsevier Science
Single-cell gel electrophoresis (comet assay) is one of the most common methods used to measure oxidatively damaged DNA in peripheral blood mononuclear cells (PBMC), as a biomarker of oxidative stress in vivo. However, storage, extraction, and assay