Modifying inter-cistronic sequence significantly enhances IRES dependent second gene expression in bicistronic vector: Construction of optimised cassette for gene therapy of familial hypercholesterolemia

Autor: Huseyin Mehmet, Wajahatullah Khan, Futwan Al-Mohanna, Zainularifeen Abduljaleel, Simon N. Waddington, Michael Themis, Charles Coutelle, Zuhair N. Al-Hassnan, S Apostolidou, Brian W. Bigger, Mohiuddin M. Taher, Mohammad Athar, Abdellatif Bouazzaoui, Mukaddes Colakogullari, Mohammed N. Al-Ahdal, Faisal A. Al-Allaf

Publikováno v: Non-coding RNA Research
Non-coding RNA Research, Vol 4, Iss 1, Pp 1-14 (2019)
Al-Allaf, F A, Abduljaleel, Z, Athar, M, Taher, M M, Khan, W, Mehmet, H, Colakogullari, M, Apostolidou, S, Bigger, B, Waddington, S, Coutelle, C, Themis, M, Al-Ahdal, M N, Al-Mohanna, F A, Al-Hassnan, Z N & Bouazzaoui, A 2019, ' Modifying inter-cistronic sequence significantly enhances IRES dependent second gene expression in bicistronic vector : Construction of optimised cassette for gene therapy of familial hypercholesterolemia ', Non-coding RNA research, vol. 4, no. 1, pp. 1-14 . https://doi.org/10.1016/j.ncrna.2018.11.005

Internal ribosome entry site (IRES) sequences have become a valuable tool in the construction of gene transfer and therapeutic vectors for multi-cistronic gene expression from a single mRNA transcript. The optimal conditions for effective use of this

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http://europepmc.org/articles/PMC6404380

Modifying inter-cistronic sequence significantly enhances IRES dependent second gene expression in bicistronic vector: Construction of optimised cassette for gene therapy of familial hypercholesterolemia.

Autor: Al-Allaf FA; Department of Medical Genetics, Faculty of Medicine, Umm Al-Qura University, P.O. Box 715, Makkah, 21955, Saudi Arabia.; Science and Technology Unit, Umm Al-Qura University, P.O. Box 715, Makkah, 21955, Saudi Arabia.; Molecular Diagnostics Unit, Department of Laboratory and Blood Bank, King Abdullah Medical City, Makkah, 21955, Saudi Arabia.; Gene Therapy Research Group, Department of Molecular and Cell Medicine, Faculty of Medicine, Imperial College London, South Kensington, London, SW7 2AZ, UK.; Institute of Reproductive and Developmental Biology, Division of Clinical Sciences, Faculty of Medicine, Imperial College London, London, W12 0NN, UK., Abduljaleel Z; Department of Medical Genetics, Faculty of Medicine, Umm Al-Qura University, P.O. Box 715, Makkah, 21955, Saudi Arabia.; Science and Technology Unit, Umm Al-Qura University, P.O. Box 715, Makkah, 21955, Saudi Arabia., Athar M; Department of Medical Genetics, Faculty of Medicine, Umm Al-Qura University, P.O. Box 715, Makkah, 21955, Saudi Arabia.; Science and Technology Unit, Umm Al-Qura University, P.O. Box 715, Makkah, 21955, Saudi Arabia., Taher MM; Department of Medical Genetics, Faculty of Medicine, Umm Al-Qura University, P.O. Box 715, Makkah, 21955, Saudi Arabia.; Science and Technology Unit, Umm Al-Qura University, P.O. Box 715, Makkah, 21955, Saudi Arabia., Khan W; Department of Basic Sciences, College of Science and Health Professions, King Saud Bin Abdulaziz University for Health Sciences, PO Box 3124, Riyadh, 11426, Saudi Arabia., Mehmet H; Institute of Reproductive and Developmental Biology, Division of Clinical Sciences, Faculty of Medicine, Imperial College London, London, W12 0NN, UK., Colakogullari M; Institute of Reproductive and Developmental Biology, Division of Clinical Sciences, Faculty of Medicine, Imperial College London, London, W12 0NN, UK., Apostolidou S; Institute of Reproductive and Developmental Biology, Division of Clinical Sciences, Faculty of Medicine, Imperial College London, London, W12 0NN, UK., Bigger B; Gene Therapy Research Group, Department of Molecular and Cell Medicine, Faculty of Medicine, Imperial College London, South Kensington, London, SW7 2AZ, UK., Waddington S; Gene Therapy Research Group, Department of Molecular and Cell Medicine, Faculty of Medicine, Imperial College London, South Kensington, London, SW7 2AZ, UK., Coutelle C; Gene Therapy Research Group, Department of Molecular and Cell Medicine, Faculty of Medicine, Imperial College London, South Kensington, London, SW7 2AZ, UK., Themis M; Gene Therapy Research Group, Department of Molecular and Cell Medicine, Faculty of Medicine, Imperial College London, South Kensington, London, SW7 2AZ, UK., Al-Ahdal MN; Department of Infection and Immunity, King Faisal Specialist Hospital & Research Center, Riyadh, 11211, Saudi Arabia., Al-Mohanna FA; Department of Cell Biology, King Faisal Specialist Hospital and Research Center, Riyadh, 11211, Saudi Arabia., Al-Hassnan ZN; Department of Medical Genetics, King Faisal Specialist Hospital and Research Center, Riyadh, 11211, Saudi Arabia., Bouazzaoui A; Department of Medical Genetics, Faculty of Medicine, Umm Al-Qura University, P.O. Box 715, Makkah, 21955, Saudi Arabia.; Science and Technology Unit, Umm Al-Qura University, P.O. Box 715, Makkah, 21955, Saudi Arabia.

Publikováno v: Non-coding RNA research [Noncoding RNA Res] 2018 Nov 22; Vol. 4 (1), pp. 1-14. Date of Electronic Publication: 2018 Nov 22 (Print Publication: 2019).