Zobrazeno 1 - 10
of 73
pro vyhledávání: '"Ines Hellmann"'
Autor:
Fiona C. Edenhofer, Anita Térmeg, Mari Ohnuki, Jessica Jocher, Zane Kliesmete, Eva Briem, Ines Hellmann, Wolfgang Enard
Publikováno v:
iScience, Vol 27, Iss 6, Pp 110090- (2024)
Summary: Comparisons of molecular phenotypes across primates provide unique information to understand human biology and evolution, and single-cell RNA-seq CRISPR interference (CRISPRi) screens are a powerful approach to analyze them. Here, we generat
Externí odkaz:
https://doaj.org/article/ef3bd4a4794a418582dfb8fad12bd644
Autor:
Philipp Janssen, Zane Kliesmete, Beate Vieth, Xian Adiconis, Sean Simmons, Jamie Marshall, Cristin McCabe, Holger Heyn, Joshua Z. Levin, Wolfgang Enard, Ines Hellmann
Publikováno v:
Genome Biology, Vol 24, Iss 1, Pp 1-22 (2023)
Abstract Background In droplet-based single-cell and single-nucleus RNA-seq experiments, not all reads associated with one cell barcode originate from the encapsulated cell. Such background noise is attributed to spillage from cell-free ambient RNA o
Externí odkaz:
https://doaj.org/article/aa9dde6a34444a78b997de85197d101b
Autor:
Zane Kliesmete, Lucas Esteban Wange, Beate Vieth, Miriam Esgleas, Jessica Radmer, Matthias Hülsmann, Johanna Geuder, Daniel Richter, Mari Ohnuki, Magdelena Götz, Ines Hellmann, Wolfgang Enard
Publikováno v:
eLife, Vol 12 (2023)
Brain size and cortical folding have increased and decreased recurrently during mammalian evolution. Identifying genetic elements whose sequence or functional properties co-evolve with these traits can provide unique information on evolutionary and d
Externí odkaz:
https://doaj.org/article/bf6727aa344644268b3d8d1a7a807590
Autor:
Christina Zeller, Daniel Richter, Vindi Jurinovic, Ilse A. Valtierra-Gutiérrez, Ashok Kumar Jayavelu, Matthias Mann, Johannes W. Bagnoli, Ines Hellmann, Tobias Herold, Wolfgang Enard, Binje Vick, Irmela Jeremias
Publikováno v:
Journal of Hematology & Oncology, Vol 15, Iss 1, Pp 1-6 (2022)
Abstract Acute myeloid leukemia (AML) patients suffer dismal prognosis upon treatment resistance. To study functional heterogeneity of resistance, we generated serially transplantable patient-derived xenograft (PDX) models from one patient with AML a
Externí odkaz:
https://doaj.org/article/ae1db258d8444cea801522faea1ea01e
Autor:
Aleksandar Janjic, Lucas E. Wange, Johannes W. Bagnoli, Johanna Geuder, Phong Nguyen, Daniel Richter, Beate Vieth, Binje Vick, Irmela Jeremias, Christoph Ziegenhain, Ines Hellmann, Wolfgang Enard
Publikováno v:
Genome Biology, Vol 23, Iss 1, Pp 1-27 (2022)
Abstract Cost-efficient library generation by early barcoding has been central in propelling single-cell RNA sequencing. Here, we optimize and validate prime-seq, an early barcoding bulk RNA-seq method. We show that it performs equivalently to TruSeq
Externí odkaz:
https://doaj.org/article/68399bd59d28488cb10eea8edd8b0b09
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-11 (2019)
There has been a rapid rise in single cell RNA-seq methods and associated pipelines. Here the authors use simulated data to systematically evaluate the performance of 3000 possible pipelines to derive recommendations for data processing and analysis
Externí odkaz:
https://doaj.org/article/2bc3c1c113b84eecbdc9f6b415ed696b
Autor:
Kazutoshi Takahashi, Michiko Nakamura, Chikako Okubo, Zane Kliesmete, Mari Ohnuki, Megumi Narita, Akira Watanabe, Mai Ueda, Yasuhiro Takashima, Ines Hellmann, Shinya Yamanaka
Publikováno v:
PLoS Genetics, Vol 17, Iss 5, p e1009587 (2021)
Human pluripotent stem cells (PSCs) express human endogenous retrovirus type-H (HERV-H), which exists as more than a thousand copies on the human genome and frequently produces chimeric transcripts as long-non-coding RNAs (lncRNAs) fused with downstr
Externí odkaz:
https://doaj.org/article/1ba3c8ef9a894d959bfe9a1a133ec14f
Autor:
Johannes W. Bagnoli, Christoph Ziegenhain, Aleksandar Janjic, Lucas E. Wange, Beate Vieth, Swati Parekh, Johanna Geuder, Ines Hellmann, Wolfgang Enard
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-8 (2018)
Single-cell RNA-barcoding and sequencing is an efficient, genome-wide method to characterize cellular identities. Here the authors systematically evaluate the protocol and develop molecular crowding SCRB-seq with improved sensitivity and cost-efficie
Externí odkaz:
https://doaj.org/article/4a131646ab114fc18a5468c079161e41
Autor:
Marion Cremer, Volker J. Schmid, Felix Kraus, Yolanda Markaki, Ines Hellmann, Andreas Maiser, Heinrich Leonhardt, Sam John, John Stamatoyannopoulos, Thomas Cremer
Publikováno v:
Epigenetics & Chromatin, Vol 10, Iss 1, Pp 1-17 (2017)
Abstract Background The association of active transcription regulatory elements (TREs) with DNAse I hypersensitivity (DHS[+]) and an ‘open’ local chromatin configuration has long been known. However, the 3D topography of TREs within the nuclear l
Externí odkaz:
https://doaj.org/article/cf677d70afed42d89fc8fced8e325c9f
Autor:
Kirk E Lohmueller, Anders Albrechtsen, Yingrui Li, Su Yeon Kim, Thorfinn Korneliussen, Nicolas Vinckenbosch, Geng Tian, Emilia Huerta-Sanchez, Alison F Feder, Niels Grarup, Torben Jørgensen, Tao Jiang, Daniel R Witte, Annelli Sandbæk, Ines Hellmann, Torsten Lauritzen, Torben Hansen, Oluf Pedersen, Jun Wang, Rasmus Nielsen
Publikováno v:
PLoS Genetics, Vol 7, Iss 10, p e1002326 (2011)
A major question in evolutionary biology is how natural selection has shaped patterns of genetic variation across the human genome. Previous work has documented a reduction in genetic diversity in regions of the genome with low recombination rates. H
Externí odkaz:
https://doaj.org/article/56808897fcc548ae9546e571d9bbcc4b