Zobrazeno 1 - 4
of 4
pro vyhledávání: '"Ines Eberhardt"'
Publikováno v:
Yeast. 21:1325-1334
Bax is a pro-apoptotic member of the Bcl-2 family of proteins involved in the regulation of genetically programmed cell death in mammalian cells. It has been shown that heterologous expression of Bax in several yeast species, such as Saccharomyces ce
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6a5d4b8116ae01515cd99d1d746f3a89
https://doi.org/10.1002/yea.1180
https://doi.org/10.1002/yea.1180
Publikováno v:
European Journal of Biochemistry. 262:191-201
In the yeast, Saccharomyces cerevisiae, pyruvate decarboxylase (Pdc) is encoded by the two isogenes PDC1 and PDC5. Deletion of the more strongly expressed PDC1 gene stimulates the promoter activity of both PDC1 and PDC5, a phenomenon called Pdc autor
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a25c549b9363c1831cd4ce170cf08e17
https://doi.org/10.1046/j.1432-1327.1999.00370.x
https://doi.org/10.1046/j.1432-1327.1999.00370.x
Publikováno v:
Biochemistry. 36:1900-1905
We investigated the importance of the interaction between the Nl'-atom of the cofactor thiamine diphosphate and glutamic acid residue 51 in pyruvate decarboxylase (EC 4.1. 1.1). The yeast wild type gene PDCl and the respective mutant genes (E51Q and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7ba08032fdec05256279d0bdec0396c6
https://doi.org/10.1021/bi961341l
https://doi.org/10.1021/bi961341l
Autor:
Ines Eberhardt, Stefan Hohmann
Publikováno v:
Current Genetics. 27:306-308
The classical disruption method for yeast genes is by using in vitro deletion of the gene of interest, or of a part of it, with restriction enzymes. We are now routinely using a strategy that takes advantage of polymerase chain reactions (PCRs) which
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5126efce77c7e359f53e6adde7248262
https://doi.org/10.1007/bf00352097
https://doi.org/10.1007/bf00352097