Zobrazeno 1 - 10
of 24
pro vyhledávání: '"Inca Ghosh"'
Publikováno v:
BioTechniques, Vol 42, Iss 1, Pp 63-69 (2007)
Synthetic peptides incorporating various chemical moieties, for example, phosphate groups, are convenient tools for investigating protein modification enzymes, such as protein phosphatases (PPs). However, short peptides are sometimes poor substrates,
Externí odkaz:
https://doaj.org/article/faeb6fe5016a4a61a559ebd56c114e9e
Publikováno v:
BioTechniques, Vol 37, Iss 3, Pp 430-443 (2004)
Peptide arrays are increasingly used to define antibody epitopes and substrate specificities of protein kinases. Their use is hampered, however, by ineffective and variable binding efficiency of peptides, which often results in low sensitivity and in
Externí odkaz:
https://doaj.org/article/63a5416e9d9b451db09b4524f58f5aac
Publikováno v:
BioTechniques, Vol 36, Iss 6, Pp 976-981 (2004)
We have applied intein-mediated peptide ligation (IPL) to the use of peptide substrates for kinase assays and subsequent Western blot analysis. IPL allows for the efficient ligation of a synthetic peptide with an N-terminal cysteine residue to an int
Externí odkaz:
https://doaj.org/article/52c527ee533b46dca30614ea32b547bc
Publikováno v:
Journal of Immunological Methods. 293:85-95
Synthetic peptides have become an important tool in antibody production and enzyme characterization. The small size of peptides, however, has hindered their use in assays systems, such as Western blots, and as immunogens. Here, we present a facile me
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6f19773a6e58da8888bb45811573b3d8
https://doi.org/10.1016/j.jim.2004.07.003
https://doi.org/10.1016/j.jim.2004.07.003
Publikováno v:
BioTechniques. 36:976-981
We have applied intein-mediated peptide ligation (IPL) to the use of peptide substrates for kinase assays and subsequent Western blot analysis. IPL allows for the efficient ligation of a synthetic peptide with an N-terminal cysteine residue to an int
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7d7576eb7b06bde197764af8c8c2e89d
https://doi.org/10.2144/04366st02
https://doi.org/10.2144/04366st02
Autor:
Ming-Qun Xu, Guillaume Lesage, Yi Ding, Zihe Rao, Inca Ghosh, Xuehui Chen, Sébastien Ferrandon
Publikováno v:
Journal of Biological Chemistry. 278:39133-39142
We have determined the crystal structure of a 154-residue intein derived from the dnaB gene of Synechocystis sp. strain PCC6803 and refined it to a 2.0-A resolution. The x-ray structure suggests that this intein possesses two catalytic sites that app
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1847e3cedf474c5c93a51fa8c17223f0
https://doi.org/10.1074/jbc.m306197200
https://doi.org/10.1074/jbc.m306197200
Publikováno v:
Journal of Biological Chemistry. 276:24051-24058
Two important aspects of protein splicing were investigated by employing the trans-splicing intein from the dnaE gene of Synechocystis sp. PCC6803. First, we demonstrated that both protein splicing and cleavage at the N-terminal splice junction were
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::70daa56df5b5f4e3652e59f82d91f82e
https://doi.org/10.1074/jbc.m011049200
https://doi.org/10.1074/jbc.m011049200
Autor:
Luo Sun, Lixin Chen, Jack Benner, Reto Kolly, Daniel Panne, Ming-Qun Xu, Xiang-Qin Liu, Thomas C. Evans, Inca Ghosh, Deana Martin
Publikováno v:
Journal of Biological Chemistry. 275:9091-9094
A naturally occurring split intein from the dnaE gene of Synechocystis sp. PCC6803 (Ssp DnaE intein) has been shown to mediate efficient in vivo and in vitro trans-splicing in a foreign protein context. A cis-splicing Ssp DnaE intein construct displa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2fd7679b7fd2a5e8652b5d6c7ae01a5c
https://doi.org/10.1074/jbc.275.13.9091
https://doi.org/10.1074/jbc.275.13.9091
Publikováno v:
Molecular and Biochemical Parasitology. 104:233-246
A screen of an expression library from the fourth larval stage (L4) of the parasitic nematode Brugia malayi resulted in the identification of a 727 bp full-length cDNA with 29–40% identity to members of the small heat shock family of proteins ( Bm
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ccd2979270d0a3601bc556ff3f73f440
https://doi.org/10.1016/s0166-6851(99)00150-4
https://doi.org/10.1016/s0166-6851(99)00150-4
Autor:
Steven A. Williams, Mark Blaxter, Wenhong Lu, Barton E. Slatko, Alan L. Scott, Inca Ghosh, David B. Guiliano, Nithyakalyani Raghavan
Publikováno v:
Molecular and Biochemical Parasitology. 77:77-93
We have used a tag sequencing approach to survey genes expressed in the third stage infective larvae of the human filarial nematode parasite Brugia malayi. RNA was isolated from late vector-stage L3 larvae after days 9 or 10 of infection in mosquitos
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7d8fb9afd5da6e7e8ec50308752f1f8f
https://doi.org/10.1016/0166-6851(96)02571-6
https://doi.org/10.1016/0166-6851(96)02571-6