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pro vyhledávání: '"In vitro protein synthesis"'
Akademický článek
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Akademický článek
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Autor:
Nawal Abd El-Baky, Maie Ahmed Elkhawaga, Eman Shawky Abdelkhalek, Mona Mohammed Sharaf, Elrashdy Mustafa Redwan, Hoda Reda Kholef
Publikováno v:
Biotechnology Reports, Vol 29, Iss , Pp e00583- (2021)
For the first time, we produced four lactoferricin (LFcin) peptides by a cell-free (in vitro) method. These short antimicrobial peptides were expressed in an E. coli cell-free protein synthesis (CFPS) system and the bioactivity of the produced peptid
Externí odkaz:
https://doaj.org/article/21f83c4910a548c1bbc555a60a1132e9
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 8 (2020)
In vitro systems are ideal setups to investigate the basic principles of biochemical reactions and subsequently the bricks of life. Cell-free protein synthesis (CFPS) systems mimic the transcription and translation processes of whole cells in a contr
Externí odkaz:
https://doaj.org/article/a8ea7fdc641e4de8ae39270103fa2546
Autor:
Nawal Abd El-Baky, Esmail M. EL-Fakharany, Soraya A. Sabry, Ehab R. El-Helow, Elrashdy Mustafa Redwan, Amira Sabry
Publikováno v:
Biology, Vol 11, Iss 2, p 157 (2022)
Cell-free (in vitro) expression is a robust alternative platform to the cell-based (in vivo) system for recombinant protein production. Tumor necrosis factor-alpha (TNF-α) is an effective pro-inflammatory cytokine with pleiotropic effects. The aim o
Externí odkaz:
https://doaj.org/article/dbe87183f911479f99346bd571ae7351
Autor:
Gutu, Nicoleta
Synthesizing proteins for use in therapeutics is restrained by, in part, contaminants in in vivo expression systems and limited production capacity of in vitro systems. Cell free expression (CFE) systems have emerged as a potential alternative for pr
Externí odkaz:
http://hdl.handle.net/10754/685593
Publikováno v:
Methods and Protocols, Vol 2, Iss 1, p 24 (2019)
Cell-free protein synthesis (CFPS) is a platform technology that provides new opportunities for protein expression, metabolic engineering, therapeutic development, education, and more. The advantages of CFPS over in vivo protein expression include it
Externí odkaz:
https://doaj.org/article/b3e4500eec584c5b95b824cf7c529d23
Publikováno v:
Data, Vol 3, Iss 4, p 41 (2018)
Protein synthesis is at the core of bottom-up construction of artificial cellular mimics. Intriguingly, several reports have revealed that when a transcription–translation (TX–TL) kit is encapsulated inside lipid vesicles (or water-in-oil droplet
Externí odkaz:
https://doaj.org/article/f33db47381e8445a80db4e1842b571e8
Autor:
Nawal Abd El-Baky, Esmail M. EL-Fakharany, Soraya A. Sabry, Ehab R. El-Helow, Elrashdy Mustafa Redwan, Amira Sabry
Publikováno v:
Biology; Volume 11; Issue 2; Pages: 157
Cell-free (in vitro) expression is a robust alternative platform to the cell-based (in vivo) system for recombinant protein production. Tumor necrosis factor-alpha (TNF-α) is an effective pro-inflammatory cytokine with pleiotropic effects. The aim o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b38a4f477a3b76cbf3f3867509312f82
https://pubmed.ncbi.nlm.nih.gov/35205024
https://pubmed.ncbi.nlm.nih.gov/35205024
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America, 1977 Nov 01. 74(11), 5104-5108.
Externí odkaz:
https://www.jstor.org/stable/67535