Porcine skeletal muscle typing in histochemical and in-situ RT-PCR analysis

Autor: Tao Lin, Zhun Liu, Fawen Dai, Hechuan Wang, Jianjun Zuo

Publikováno v: Veterinary and Animal Science, Vol 25, Iss , Pp 100332- (2024)

Currently, there are plenty of histochemical methods to classify pig muscle fibers, which confused the naming and classification of muscle fibers. This study aims to analyze the difference and correlation of 6 different histochemical methods and sele

Externí odkaz: https://doaj.org/article/ac74550f28b449ae9c9a18c94dc56ae7

An optimized protocol for detecting guard cell specific gene expression by in situ RT-PCR in Brassica rapa

Autor: Yingying Song, Xinlei Guo, Jian Wu, Jianli Liang, Runmao Lin, Zifu Yan, Xiaowu Wang

Publikováno v: Horticultural Plant Journal, Vol 8, Iss 3, Pp 311-318 (2022)

It is important to detect specific genes expressed in the guard cells, which control gas exchange and play key roles in response to drought and salt stresses. Due to the genetic transformation of Chinese cabbage (Brassica rapa) has not been well deve

Externí odkaz: https://doaj.org/article/d747933598ea48cda0be32e041ad3350

An optimized protocol for detecting guard cell specific gene expression by in situ RT-PCR in Brassica rapa

Autor: Song, Yingying, Guo, Xinlei, Wu, Jian, Liang, Jianli, Lin, Runmao, Yan, Zifu, Wang, Xiaowu

Publikováno v: In Horticultural Plant Journal May 2022 8(3):311-318

A Novel Method for In Situ RT-PCR Based on Capsules from Centrifuge Tubes, Ideal for Transcripts Detection in Plant Tissues.

Autor: Laines-Hidalgo JI; Unidad de Biología Integrativa, Centro de Investigación Científica de Yucatán, Merida, Yucatán, Mexico., Kú-González AF; Unidad de Biología Integrativa, Centro de Investigación Científica de Yucatán, Merida, Yucatán, Mexico., Vázquez-Flota FA; Unidad de Biología Integrativa, Centro de Investigación Científica de Yucatán, Merida, Yucatán, Mexico. felipe@cicy.mx.

Publikováno v: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2827, pp. 417-433.

Detection of Mycobacterium avium spp. paratuberculosis (Map) in samples of sheep paratuberculosis (Johne's disease or JD) and human Crohn's disease (CD) using liquid phase RT-PCR, in situ RT-PCR and immunohistochemistry

Autor: Rocca, S., Cubeddu, T., Nieddu, A.M., Pirino, S., Appino, S., Antuofermo, E., Tanda, F., Verin, R., Sechi, L.A., Taccini, E., Leoni, A.

Publikováno v: In Small Ruminant Research 2010 88(2):126-134