Improved protocol for Bst polymerase and reverse transcriptase production and application to a point-of-care diagnostics system.

Autor: de Souza LR; Center for Molecular Biology and Genetic Engineering (CBMEG), Universidade Estadual de Campinas (UNICAMP), Campinas/SP 13083-875, Brazil.; Center for Medicinal Chemistry (CQMED), Universidade Estadual de Campinas (UNICAMP), Campinas/SP 13083-886, Brazil., Silva IEPD; Center for Molecular Biology and Genetic Engineering (CBMEG), Universidade Estadual de Campinas (UNICAMP), Campinas/SP 13083-875, Brazil.; Center for Medicinal Chemistry (CQMED), Universidade Estadual de Campinas (UNICAMP), Campinas/SP 13083-886, Brazil.; Department of Biochemistry and Tissue Biology, Institute of Biology, Universidade Estadual de Campinas (UNICAMP), Campinas/SP 13083-862, Brazil., Celis-Silva G; Center for Molecular Biology and Genetic Engineering (CBMEG), Universidade Estadual de Campinas (UNICAMP), Campinas/SP 13083-875, Brazil.; Center for Medicinal Chemistry (CQMED), Universidade Estadual de Campinas (UNICAMP), Campinas/SP 13083-886, Brazil., Raddatz BW; Hilab, Curitiba/PR 81270-185, Brazil., Imamura LM; Hilab, Curitiba/PR 81270-185, Brazil., Kim EYS; Hilab, Curitiba/PR 81270-185, Brazil., Valderrama GV; Center for Molecular Biology and Genetic Engineering (CBMEG), Universidade Estadual de Campinas (UNICAMP), Campinas/SP 13083-875, Brazil.; Center for Medicinal Chemistry (CQMED), Universidade Estadual de Campinas (UNICAMP), Campinas/SP 13083-886, Brazil., Riedi HP; Hilab, Curitiba/PR 81270-185, Brazil., Rogal SR Jr; Hilab, Curitiba/PR 81270-185, Brazil., Almeida BMM; Hilab, Curitiba/PR 81270-185, Brazil., Figueredo MVM; Hilab, Curitiba/PR 81270-185, Brazil., Bengtson MH; Center for Medicinal Chemistry (CQMED), Universidade Estadual de Campinas (UNICAMP), Campinas/SP 13083-886, Brazil.; Department of Biochemistry and Tissue Biology, Institute of Biology, Universidade Estadual de Campinas (UNICAMP), Campinas/SP 13083-862, Brazil., Massirer KB; Center for Molecular Biology and Genetic Engineering (CBMEG), Universidade Estadual de Campinas (UNICAMP), Campinas/SP 13083-875, Brazil.; Center for Medicinal Chemistry (CQMED), Universidade Estadual de Campinas (UNICAMP), Campinas/SP 13083-886, Brazil.

Publikováno v: Experimental biology and medicine (Maywood, N.J.) [Exp Biol Med (Maywood)] 2023 Oct; Vol. 248 (19), pp. 1671-1683. Date of Electronic Publication: 2023 Dec 13.

Clinical Validation of a Colorimetric Loop-Mediated Isothermal Amplification Using a Portable Device for the Rapid Detection of SARS-CoV-2.

Autor: Raddatz BW; Hilab, Rua José Altair Possebom, 800-CIC, Curitiba 81270-185, PR, Brazil., Rabello FJ; Hilab, Rua José Altair Possebom, 800-CIC, Curitiba 81270-185, PR, Brazil., Benedetti R; Hilab, Rua José Altair Possebom, 800-CIC, Curitiba 81270-185, PR, Brazil., Steil GJ; Hilab, Rua José Altair Possebom, 800-CIC, Curitiba 81270-185, PR, Brazil., Imamura LM; Hilab, Rua José Altair Possebom, 800-CIC, Curitiba 81270-185, PR, Brazil., Kim EYS; Hilab, Rua José Altair Possebom, 800-CIC, Curitiba 81270-185, PR, Brazil., Santiago EB; Hilab, Rua José Altair Possebom, 800-CIC, Curitiba 81270-185, PR, Brazil., Hartmann LF; Hilab, Rua José Altair Possebom, 800-CIC, Curitiba 81270-185, PR, Brazil., Predebon JV; Hilab, Rua José Altair Possebom, 800-CIC, Curitiba 81270-185, PR, Brazil., Delfino BM; Hilab, Rua José Altair Possebom, 800-CIC, Curitiba 81270-185, PR, Brazil., Nogueira MB; Virology Laboratory, Universidade Federal do Paraná (Hospital de Clínicas), Rua General Carneiro, 181-Alto da Glória, Curitiba 80060-900, PR, Brazil., Dos Santos JS; Virology Laboratory, Universidade Federal do Paraná (Hospital de Clínicas), Rua General Carneiro, 181-Alto da Glória, Curitiba 80060-900, PR, Brazil., da Silva BG; Virology Laboratory, Universidade Federal do Paraná (Hospital de Clínicas), Rua General Carneiro, 181-Alto da Glória, Curitiba 80060-900, PR, Brazil., Nicollete DRP; Hilab, Rua José Altair Possebom, 800-CIC, Curitiba 81270-185, PR, Brazil., Almeida BMM; Hilab, Rua José Altair Possebom, 800-CIC, Curitiba 81270-185, PR, Brazil., Rogal SR Jr; Hilab, Rua José Altair Possebom, 800-CIC, Curitiba 81270-185, PR, Brazil., Figueredo MVM; Hilab, Rua José Altair Possebom, 800-CIC, Curitiba 81270-185, PR, Brazil.

Publikováno v: Diagnostics (Basel, Switzerland) [Diagnostics (Basel)] 2023 Apr 06; Vol. 13 (7). Date of Electronic Publication: 2023 Apr 06.

A blocking antibody against canine CSF-1R maturated by limited CDR mutagenesis.

Autor: Beirão BCB; The Royal (Dick) School of Veterinary Studies and Roslin Institute, The University of Edinburgh-Easter Bush, Midlothian, EH25 9RG, UK., Raposo TP; The Royal (Dick) School of Veterinary Studies and Roslin Institute, The University of Edinburgh-Easter Bush, Midlothian, EH25 9RG, UK., Imamura LM; Department of Research and Development, Imunova Análises Biológicas, Curitiba, PR 80215-182, Brazil., Ingberman M; Department of Research and Development, Imunova Análises Biológicas, Curitiba, PR 80215-182, Brazil., Hupp T; Cancer Research UK Edinburgh Centre MRC Institute of Genetics & Molecular Medicine, Western General Hospital, The University of Edinburgh, Edinburgh, EH4 2XR, UK., Vojtěšek B; Regional Centre for Applied Molecular Oncology, Masaryk Memorial Cancer Institute, Brno, 656 53, Czech Republic., Argyle DJ; The Royal (Dick) School of Veterinary Studies and Roslin Institute, The University of Edinburgh-Easter Bush, Midlothian, EH25 9RG, UK.

Publikováno v: Antibody therapeutics [Antib Ther] 2020 Aug 12; Vol. 3 (3), pp. 193-204. Date of Electronic Publication: 2020 Aug 12 (Print Publication: 2020).