Zobrazeno 1 - 10
of 133
pro vyhledávání: '"Ilya J. Finkelstein"'
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-12 (2024)
Abstract CASTs use both CRISPR-associated proteins and Tn7-family transposons for RNA-guided vertical and horizontal transmission. CASTs encode minimal CRISPR arrays but can’t acquire new spacers. Here, we report that CASTs can co-opt defense-assoc
Externí odkaz:
https://doaj.org/article/87ad148d25754558b864c70a17ead820
Autor:
Pedro Leão, Mary E. Little, Kathryn E. Appler, Daphne Sahaya, Emily Aguilar-Pine, Kathryn Currie, Ilya J. Finkelstein, Valerie De Anda, Brett J. Baker
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-9 (2024)
Abstract Dozens of new antiviral systems have been recently characterized in bacteria. Some of these systems are present in eukaryotes and appear to have originated in prokaryotes, but little is known about these defense mechanisms in archaea. Here,
Externí odkaz:
https://doaj.org/article/06444ba84f164fc78b7f8db7ff8e556e
Autor:
Grace N. Hibshman, Jack P. K. Bravo, Matthew M. Hooper, Tyler L. Dangerfield, Hongshan Zhang, Ilya J. Finkelstein, Kenneth A. Johnson, David W. Taylor
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-15 (2024)
Abstract CRISPR-Cas9 is a powerful tool for genome editing, but the strict requirement for an NGG protospacer-adjacent motif (PAM) sequence immediately next to the DNA target limits the number of editable genes. Recently developed Cas9 variants have
Externí odkaz:
https://doaj.org/article/bcc8918303d84f72875bedfc0ceaf526
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-13 (2024)
Abstract CRISPR-Cas13d cleaves RNA and is used in vivo and for diagnostics. However, a systematic understanding of its RNA binding and cleavage specificity is lacking. Here, we describe an RNA Chip-Hybridized Association-Mapping Platform (RNA-CHAMP)
Externí odkaz:
https://doaj.org/article/707aad093e864c7e8d26b0eba435ea43
Autor:
Behrouz Eslami-Mossallam, Misha Klein, Constantijn V. D. Smagt, Koen V. D. Sanden, Stephen K. Jones, John A. Hawkins, Ilya J. Finkelstein, Martin Depken
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-10 (2022)
Cas9 off-target sites can be predicted by many bioinformatics tools. Here the authors present low complexity mechanistic model that characterizes SpCas9 kinetics in free-energy terms, allowing quantitative prediction of off-target activity in bulk-bi
Externí odkaz:
https://doaj.org/article/8ce17f4dfe3843928f961470b5546a13
Autor:
Marie-Christine Caron, Ajit K. Sharma, Julia O’Sullivan, Logan R. Myler, Maria Tedim Ferreira, Amélie Rodrigue, Yan Coulombe, Chantal Ethier, Jean-Philippe Gagné, Marie-France Langelier, John M. Pascal, Ilya J. Finkelstein, Michael J. Hendzel, Guy G. Poirier, Jean-Yves Masson
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-16 (2019)
Poly(ADP-ribose) polymerase-1 (PARP-1) facilitates local chromatin relaxation and the recruitment of DNA repair factors at double strand breaks site (DSBs). Here the authors reveal that PARP-1 acts as a critical regulator of DNA end resection of DSBs
Externí odkaz:
https://doaj.org/article/9f5d756d157a42a383cdfc2eb5b3e5f7
Autor:
S. Wesley Long, Randall J. Olsen, Paul A. Christensen, David W. Bernard, James J. Davis, Maulik Shukla, Marcus Nguyen, Matthew Ojeda Saavedra, Prasanti Yerramilli, Layne Pruitt, Sishir Subedi, Hung-Che Kuo, Heather Hendrickson, Ghazaleh Eskandari, Hoang A. T. Nguyen, J. Hunter Long, Muthiah Kumaraswami, Jule Goike, Daniel Boutz, Jimmy Gollihar, Jason S. McLellan, Chia-Wei Chou, Kamyab Javanmardi, Ilya J. Finkelstein, James M. Musser
Publikováno v:
mBio, Vol 11, Iss 6 (2020)
ABSTRACT We sequenced the genomes of 5,085 severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) strains causing two coronavirus disease 2019 (COVID-19) disease waves in metropolitan Houston, TX, an ethnically diverse region with 7 million res
Externí odkaz:
https://doaj.org/article/9f1850b41a7e45ea80ff416602d5d71c
Autor:
Ja-Hwan Seol, Cory Holland, Xiaolei Li, Christopher Kim, Fuyang Li, Melisa Medina-Rivera, Robin Eichmiller, Ignacio F. Gallardo, Ilya J. Finkelstein, Paul Hasty, Eun Yong Shim, Jennifer A. Surtees, Sang Eun Lee
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-13 (2018)
The yeast Rad1–Rad10 complex has multiple roles in DNA damage repair. Here the authors uncover mutants that uncouple the roles in UV excision repair and non-NER functions.
Externí odkaz:
https://doaj.org/article/1aa8a55b041d446c9bd15181548a0127
Publikováno v:
Scientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
Abstract Single-molecule studies of protein-nucleic acid interactions frequently require site-specific modification of long DNA substrates. The bacteriophage λ is a convenient source of high quality long (48.5 kb) DNA. However, introducing specific
Externí odkaz:
https://doaj.org/article/8b70cb40cf1a47ca9a485db753930e3d
Autor:
Anna L. Mallam, Wisath Sae-Lee, Jeffrey M. Schaub, Fan Tu, Anna Battenhouse, Yu Jin Jang, Jonghwan Kim, John B. Wallingford, Ilya J. Finkelstein, Edward M. Marcotte, Kevin Drew
Publikováno v:
Cell Reports, Vol 29, Iss 5, Pp 1351-1368.e5 (2019)
Summary: RNA-binding proteins (RBPs) play essential roles in biology and are frequently associated with human disease. Although recent studies have systematically identified individual RNA-binding proteins, their higher-order assembly into ribonucleo
Externí odkaz:
https://doaj.org/article/e8a21dcd8f5c43018b9c8767abe0acbb