Zobrazeno 1 - 10
of 39
pro vyhledávání: '"Ilkka Lähdesmäki"'
Publikováno v:
Sensors and Actuators B: Chemical. 162:128-134
The tear film is a valuable diagnostic fluid for a continuous, minimally invasive surveillance of health conditions in a patient. We developed an electronic enzymatic l -lactate sensor on a polymer substrate molded into contact lens shape for potenti
Publikováno v:
Microsystem Technologies. 17:1505-1510
We demonstrate the direct immobilization of glucose oxidase and lactate oxidase onto photoresists commonly used in microfabrication. The method allows for a cost-effective, facile inclusion of enzyme functionality into novel MEMS devices because it d
Publikováno v:
Microsystem Technologies. 17:1511-1518
We present the fabrication and characterization of nanoscale fluidic channels with embedded electrodes. Arrays of 2.25 μm long and 60 nm tall nanochannels with widths ranging from 60 to 500 nm were microfabricated in SiO2 with Au electrodes embedded
Publikováno v:
IEEE Instrumentation & Measurement Magazine. 13:14-17
This article discusses about the development of a range of fabrication technologies that enable the construction of a sophisticated system for the detection of glucose from the tear film. It also demonstrates the operation of a sensor with enough sen
Publikováno v:
The Analyst. 132:818-822
This work comprises the development of a technique for the capture of single-stranded DNA on a solid support combined with in situ quantification. The capture is based on the strong and selective interaction between biotinylated DNA and streptavidin-
Publikováno v:
The Analyst. 132:811-817
This paper describes a method for monitoring the degradation of hydrogen peroxide by cells immobilized on a beaded support. The detection is based on the voltammetric reduction of hydrogen peroxide on a mercury film working electrode, whilst combinin
Novel flow injection methods for drug-receptor interaction studies, based on probing cell metabolism
Publikováno v:
The Analyst. 125:1889-1895
Publikováno v:
Trends in Biotechnology. 17:443-447
Bead-injection spectroscopy is a novel technique that uses immobilized eukaryotic cells on microbeads as a renewable biosensor for fluorescence microscopy. The use of a flow injection instrument allows fast functional assays that generate full kineti
Publikováno v:
Analytical Chemistry. 71:5248-5252
This paper describes a method for detecting oxygen consumption of adherent cell cultures. The sensing is based on oxygen-dependent quenching of the phosphorescence of a Pt-porphyrin complex immobilized on microcarrier beads, which are used as the cel
Autor:
Ilkka Lähdesmäki, Jaromir Ruzicka
Publikováno v:
Fresenius' Journal of Analytical Chemistry. 362:67-72
Flow Injection Fluorescence Microscopy (FIFM) has been further developed to become a useful tool for a multiparametric study of the response of living cells to a biochemical stimulus. Rather than recording several parameters simultaneously, the multi