Zobrazeno 1 - 7
of 7
pro vyhledávání: '"Ilka V. Thiel"'
Autor:
Kristina Friedel, Monika A. Popp, Julian C. J. Matern, Emerich M. Gazdag, Ilka V. Thiel, Gerrit Volkmann, Wulf Blankenfeldt, Henning D. Mootz
Publikováno v:
Chemical Science
Steric bulk can compensate for a catalytically critical histidine in an intein's active site and promote the N–S acyl shift.
Inteins remove themselves from a precursor protein by protein splicing. Due to the concomitant structural changes of t
Inteins remove themselves from a precursor protein by protein splicing. Due to the concomitant structural changes of t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::14384406f970557b02759cb6e411a35e
https://hdl.handle.net/10033/621664
https://hdl.handle.net/10033/621664
Publikováno v:
Angewandte Chemie. 126:1330-1334
Protein trans-splicing catalyzed by split inteins is a powerful technique for assembling a polypeptide backbone from two separate parts. However, split inteins with robust efficiencies and short fragments suitable for peptide synthesis are rare and h
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::25290f570f0068c172e588e1e3648add
https://doi.org/10.1002/ange.201307969
https://doi.org/10.1002/ange.201307969
Publikováno v:
Biochemistry. 51:233-242
Inteins are single-turnover catalysts that splice themselves out of a precursor polypeptide chain. For most inteins, the first step of protein splicing is the formation of a thioester through an N-S acyl shift at the upstream splice junction. However
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::74f26116557ec7df1b21729f416d350a
https://doi.org/10.1021/bi2014823
https://doi.org/10.1021/bi2014823
Publikováno v:
Journal of Biological Chemistry. 286:34440-34447
Inteins are internal protein sequences that post-translationally self-excise and splice together the flanking sequences, the so-called exteins. Natural and engineered inteins have been used in many practical applications. However, inteins are often i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::310760f7a07c1a7d26fec9505a200db7
https://doi.org/10.1074/jbc.m111.277350
https://doi.org/10.1074/jbc.m111.277350
Publikováno v:
Journal of Peptide Science. 16:575-581
Split inteins link their fused peptide or protein sequences with a peptide bond in an autocatalytic reaction called protein trans-splicing. This reaction is becoming increasingly important for a variety of applications in protein semisynthesis, polyp
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c958ee70f158afa76a40a6dcd00241b9
https://doi.org/10.1002/psc.1243
https://doi.org/10.1002/psc.1243
Autor:
Julian C J, Matern, Anne-Lena, Bachmann, Ilka V, Thiel, Gerrit, Volkmann, Alexandra, Wasmuth, Jens, Binschik, Henning D, Mootz
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1266
Protein trans-splicing using split inteins is a powerful and convenient reaction to chemically modify recombinantly expressed proteins under mild conditions. In particular, semisynthetic protein trans-splicing with one intein fragment short enough to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::716c9c2493df3170e107d957d826ad9b
https://pubmed.ncbi.nlm.nih.gov/25560072
https://pubmed.ncbi.nlm.nih.gov/25560072
Autor:
Gerrit Volkmann, Jens Binschik, Anne-Lena Bachmann, Henning D. Mootz, Alexandra Wasmuth, Ilka V. Thiel, Julian C. J. Matern
Publikováno v:
Site-Specific Protein Labeling ISBN: 9781493922710
Protein trans-splicing using split inteins is a powerful and convenient reaction to chemically modify recombinantly expressed proteins under mild conditions. In particular, semisynthetic protein trans-splicing with one intein fragment short enough to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::bb2b6bca97bacf3b796f99e1084234dd
https://doi.org/10.1007/978-1-4939-2272-7_9
https://doi.org/10.1007/978-1-4939-2272-7_9