Higher order scaffoldin assembly in Ruminococcus flavefaciens cellulosome is coordinated by a discrete cohesin-dockerin interaction

Autor: Pedro Bule, Virgínia M. R. Pires, Victor D. Alves, Ana Luísa Carvalho, José A. M. Prates, Luís M. A. Ferreira, Steven P. Smith, Harry J. Gilbert, Ilit Noach, Edward A. Bayer, Shabir Najmudin, Carlos M. G. A. Fontes

Publikováno v: Scientific Reports, Vol 8, Iss 1, Pp 1-14 (2018)

Abstract Cellulosomes are highly sophisticated molecular nanomachines that participate in the deconstruction of complex polysaccharides, notably cellulose and hemicellulose. Cellulosomal assembly is orchestrated by the interaction of enzyme-borne doc

Externí odkaz: https://doaj.org/article/1a033850155d48b8a516196cee73f64c

Architecturally complex O -glycopeptidases are customized for mucin recognition and hydrolysis

Autor: Ilit Noach, Alisdair B. Boraston, Elizabeth Ficko-Blean, Christopher P. Stuart, Warren W. Wakarchuk, Nakita Buenbrazo, Benjamin Pluvinage, Nicole K. Thompson, Hayden McClure

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America
Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2021, 118 (10), pp.e2019220118. ⟨10.1073/pnas.2019220118⟩

International audience; A challenge faced by peptidases is the recognition of highly diverse substrates. A feature of some peptidase families is the capacity to specifically use post-translationally added glycans present on their protein substrates a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::190722fc37c4554cffe37e4ad575ebc5
https://doi.org/10.1073/pnas.2019220118

Recognition of protein-linked glycans as a determinant of peptidase activity

Autor: Nakita Buenbrazo, Alisdair B. Boraston, Warren W. Wakarchuk, Benjamin Pluvinage, John E. Burke, Meredith L. Jenkins, Michel Gilbert, Denis Brochu, Christopher P. Stuart, Elizabeth Ficko-Blean, Ilit Noach

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America
Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2017, 114 (5), pp.E679-E688. ⟨10.1073/pnas.1615141114⟩

The vast majority of proteins are posttranslationally altered, with the addition of covalently linked sugars (glycosylation) being one of the most abundant modifications. However, despite the hydrolysis of protein peptide bonds by peptidases being a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a9c5b4dcf75ff2e802342b732fd9290
https://doi.org/10.1073/pnas.1615141114

Structural and functional characterization of a novel type-III dockerin fromRuminococcus flavefaciens

Autor: Ilit Noach, Maroor K. Jobby, Alon Karpol, Steven P. Smith, Edward A. Bayer, Michal Slutzki, Seth Chitayat

Publikováno v: FEBS Letters. 587:30-36

Phylogenetic analysis of known dockerins in Ruminococcus flavefaciens revealed a novel subtype, type-Ill, in the scaffoldin proteins, ScaA, ScaB, ScaC and ScaE. In this study, we explored the Ca 2+ -binding properties of the type-Ill dockerin from th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3995e2ca141ac4a09eefa1e7569b4810
https://doi.org/10.1016/j.febslet.2012.11.012

Homology swapping of intrinsic secondary structural elements between cellulosomal types I and II cohesins and their effect on dockerin binding

Autor: Ilit Noach, Felix Frolow, Raphael Lamed, Edward A. Bayer, Yoav Barak

Publikováno v: Pure and Applied Chemistry. 82:193-204

The high-affinity cohesin–dockerin interaction dictates the suprastructural assembly of the multienzyme cellulosome complex. The interaction between these two complementary families of protein modules was found to be species-specific and type-depen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::8530bf3e17dabc39851eba2f7240e457
https://doi.org/10.1351/pac-con-09-02-11