Targeted deletion of grape retrotransposon associated with fruit skin color via CRISPR/Cas9 in Vitis labrascana 'Shine Muscat'.

Autor: Ikuko Nakajima, Hiroyuki Kawahigashi, Chikako Nishitani, Akifumi Azuma, Takashi Haji, Seiichi Toki, Masaki Endo

Publikováno v: PLoS ONE, Vol 18, Iss 6, p e0286698 (2023)

Transposition of transposable elements affect expression levels, splicing and epigenetic status, and function of genes located in, or near, the inserted/excised locus. For example, in grape, presence of the Gret1 retrotransposon in the promoter regio

Externí odkaz: https://doaj.org/article/915c201679184566b9299a5a0fc93d1a

Embryogenic callus induction and Agrobacterium-mediated genetic transformation of ‘Shine Muscat’ grape

Autor: Ikuko Nakajima, Masaki Endo, Toshiya Yamamoto, Takaya Moriguchi, Takashi Haji

Publikováno v: Plant Biotechnology. 37:185-194

We established a method for embryogenic callus induction and highly efficient Agrobacterium-mediated genetic transformation of a table grape cultivar 'Shine Muscat' (Vitis labruscana). Embryogenic calli were induced using flower bud filaments from a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::c91864227cfbe429b825fa0c565a5c3a
https://doi.org/10.5511/plantbiotechnology.20.0527a

Effects of GA3+4 and GA4+7 Application Either Alone or Combined with Prohexadione-Ca on Fruit Development of Japanese Pear ‘Kosui’

Autor: Takaaki Nishijima, Akiko Ito, Takaya Moriguchi, Akihiro Itai, Yuri Nakamura, Daisuke Sakamoto, Naomi Oyama-Okubo, Ikuko Nakajima

Publikováno v: The Horticulture Journal. 85:201-208

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::0ac1ba7521913ded4c2da949ddbb2029
https://doi.org/10.2503/hortj.mi-107

Agrobacterium-mediated genetic transformation using cotyledons in Japanese pear (Pyrus pyrifolia).

Autor: Ikuko Nakajima^1,2, Yoshihiko Sato², Toshihiro Saito², Takaya Moriguchi^1,2, Toshiya Yamamoto^1,2 toshiya@affrc.go.jp

Publikováno v: Breeding Science. Sep2013, Vol. 63 Issue 3, p275-283. 9p.

CRISPR/Cas9-mediated targeted mutagenesis in grape

Autor: Seiichi Toki, Masaki Endo, Takaya Moriguchi, Akifumi Azuma, Toshiya Yamamoto, Ikuko Nakajima, Yusuke Ban, Noriyuki Onoue

Publikováno v: PLoS ONE
SC30201804130039
NARO成果DBa
This is an open access article distributed under the terms of the Creative Commons Attribution License. CC BY 4.0
PLoS ONE, Vol 12, Iss 5, p e0177966 (2017)
書誌情報のみ

RNA-guided genome editing using the CRISPR/Cas9 CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 (CRISPR-associated protein 9) system has been applied successfully in several plant species. However, to date, there are few repor

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::be0afc1957282dcfd3fdfe116377240a
https://pubmed.ncbi.nlm.nih.gov/28542349