Zobrazeno 1 - 3
of 3
pro vyhledávání: '"Ikenna C Okafor"'
Publikováno v:
Methods. 204:319-326
Like helicases, CRISPR proteins such as Cas9 and Cas12a unwind DNA, but unlike helicases, these CRISPR proteins do not use ATP. Instead, they use binding energy to melt DNA locally and then utilize basepairing between guide (g) RNA and target strand
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::355f8e8753397a40358d47da7391c8d0
https://doi.org/10.1016/j.ymeth.2021.11.003
https://doi.org/10.1016/j.ymeth.2021.11.003
Autor:
Ikenna C. Okafor, Taekjip Ha
Publikováno v:
The journal of physical chemistry. B.
CRISPR Cas9 is an RNA guided endonuclease that is part of a bacterial adaptive immune system. Single guide RNA (sgRNA) can be designed to target genomic DNA, making Cas9 a programmable DNA binding/cutting enzyme and allowing applications such as epig
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2298eddb6cc3bf4c8e74355cf35999d9
https://pubmed.ncbi.nlm.nih.gov/36563314
https://pubmed.ncbi.nlm.nih.gov/36563314
Autor:
Yanbo Wang, Minhee Jung, Taekjip Ha, Jungjoon K. Lee, Scott Bailey, Digvijay Singh, John Mallon, Ikenna C Okafor, Haobo Wang
Publikováno v:
Nucleic Acids Research
Cas9 has made a wide range of genomic manipulation possible. However, its specificity continues to be a challenge. Non-canonical gRNAs and new engineered variants of Cas9 have been developed to improve specificity, but at the cost of the on-target ac
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3cc1a81570f9928f8cb73cc90029a715
https://doi.org/10.1093/nar/gkz1058
https://doi.org/10.1093/nar/gkz1058