Zobrazeno 1 - 10
of 28
pro vyhledávání: '"Igor G. L. Libourel"'
Autor:
Hong Yang, Elias W Krumholz, Evan D Brutinel, Nagendra P Palani, Michael J Sadowsky, Andrew M Odlyzko, Jeffrey A Gralnick, Igor G L Libourel
Publikováno v:
PLoS Computational Biology, Vol 10, Iss 9, p e1003848 (2014)
Transposon mutagenesis, in combination with parallel sequencing, is becoming a powerful tool for en-masse mutant analysis. A probability generating function was used to explain observed miniHimar transposon insertion patterns, and gene essentiality c
Externí odkaz:
https://doaj.org/article/07abc409acf04af58d95ec660176f4de
Publikováno v:
PLoS ONE, Vol 9, Iss 3, p e91537 (2014)
Phenotype in multicellular organisms is the consequence of dynamic metabolic events that occur in a spatially dependent fashion. This spatial and temporal complexity presents challenges for investigating metabolism; creating a need for improved metho
Externí odkaz:
https://doaj.org/article/cc9b3ce7d0a44a2aacadd09b8de3e2ad
Autor:
David A Burdge, Igor G L Libourel
Publikováno v:
PLoS ONE, Vol 9, Iss 3, p e92108 (2014)
Bioreactors are designed to support highly controlled environments for growth of tissues, cell cultures or microbial cultures. A variety of bioreactors are commercially available, often including sophisticated software to enhance the functionality of
Externí odkaz:
https://doaj.org/article/90c6434de52f4913a81ad54c1404fe6b
Sequence-based Network Completion Reveals the Integrality of Missing Reactions in Metabolic Networks
Autor:
Igor G. L. Libourel, Elias W. Krumholz
Publikováno v:
Journal of Biological Chemistry. 290:19197-19207
Genome-scale metabolic models are central in connecting genotypes to metabolic phenotypes. However, even for well studied organisms, such as Escherichia coli, draft networks do not contain a complete biochemical network. Missing reactions are referre
Publikováno v:
The Plant Journal. 77:476-486
¹³C metabolic flux analysis (MFA) has become the experimental method of choice to investigate the cellular metabolism of microbes, cell cultures and plant seeds. Conventional steady-state MFA utilizes isotopic labeling measurements of amino acids o
Autor:
Igor G. L. Libourel, Elias W. Krumholz
Publikováno v:
Biophysical journal. 113(3)
In pursuit of establishing a realistic metabolic phenotypic space, the reversibility of reactions is thermodynamically constrained in modern metabolic networks. The reversibility constraints follow from heuristic thermodynamic poise approximations th
Publikováno v:
Journal of microbiological methods. 133
Site specific recombinases are invaluable tools in molecular biology, and are emerging as powerful recorders of cellular events in synthetic biology. We have developed a stringently controlled FLP recombinase system in Escherichia coli using an arabi
The effects of root anoxia on a tobacco (Nicotiana tabacum) wild type (WT) and a transformant (LNR-H) lacking root nitrate reductase were compared. LNR-H plants were visibly more sensitive to oxygen deprivation than WT, showing rapid and heavy wiltin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d643bb4edb06e0e50bb30985ce0e919b
https://doi.org/10.1023/a:1024591116697
https://doi.org/10.1023/a:1024591116697
Publikováno v:
Analytical chemistry. 88(11)
In vivo isotopic labeling coupled with high-resolution proteomics is used to investigate primary metabolism in techniques such as stable isotope probing (protein-SIP) and peptide-based metabolic flux analysis (PMFA). Isotopic enrichment of carbon sub
Autor:
Igor G. L. Libourel, Yair Shachar-Hill
Publikováno v:
Annual Review of Plant Biology. 59:625-650
Metabolic flux analysis (MFA) is a rapidly developing field concerned with the quantification and understanding of metabolism at the systems level. The application of MFA has produced detailed maps of flow through metabolic networks of a range of pla