Selection and Optimization of Medium Components for the Efficient Production of L-Asparaginase by Leucosporidium scottii L115—A Psychrotolerant Yeast

Autor: Ignacio S. Moguel, Celina K. Yamakawa, Larissa P. Brumano, Adalberto Pessoa, Solange I. Mussatto

Publikováno v: Fermentation, Vol 8, Iss 8, p 398 (2022)

This study reports the production of L-asparaginase (ASNase), an enzyme mainly used for the treatment of acute lymphoblastic leukemia, by Leucosporidiumscottii L115, a psychrotolerant yeast isolated from the Antarctic ecosystem. Focus was given to se

Externí odkaz: https://doaj.org/article/176c7f024f104ae3a1153ec7a0c82dc1

L-asparaginase Production by Leucosporidium scottii in a Bench-Scale Bioreactor With Co-production of Lipids

Autor: Adalberto Pessoa, Solange I. Mussatto, Celina Kiyomi Yamakawa, Ignacio S. Moguel

Publikováno v: Frontiers in Bioengineering and Biotechnology, Vol 8 (2020)
Frontiers in Bioengineering and Biotechnology
Moguel, I S, Yamakawa, C K, Pessoa, A & Mussatto, S I 2020, ' L-asparaginase Production by Leucosporidium scottii in a Bench-Scale Bioreactor With Co-production of Lipids ', Frontiers in Bioengineering and Biotechnology, vol. 8, 576511 . https://doi.org/10.3389/fbioe.2020.576511

L-asparaginase (ASNase) is a therapeutical enzyme used for treatment of acute lymphoblastic leukemia. ASNase products available in the market are produced by bacteria and usually present allergic response and important toxicity effects to the patient

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::12b1783c9540d4720bf0a5d4ae61a2de
https://www.frontiersin.org/articles/10.3389/fbioe.2020.576511/full

Improvement in extracellular secretion of recombinant L-asparaginase II by Escherichia coli BL21 (DE3) using glycine and n-dodecane

Autor: Gisele Monteiro, Adalberto Pessoa, Juan Carlos Flores-Santos, Michele Vitolo, Ignacio S. Moguel

Publikováno v: Braz J Microbiol
Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP

l-asparaginase II (ASNase) is the biopharmaceutical of choice for the treatment of acute lymphoblastic leukaemia. In this study, E. coli BL21 (DE3) transformed with the pET15b + asnB vector which expresses recombinant ASNase was used as a source to o

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0fcf3d13e464e30e074bb8edefb9bf8c
https://pubmed.ncbi.nlm.nih.gov/34100260