Zobrazeno 1 - 7
of 7
pro vyhledávání: '"Ignacio Asial"'
Autor:
Yuri Frosi, Yen-Chu Lin, Jiang Shimin, Siti Radhiah Ramlan, Kelly Hew, Alf Henrik Engman, Anil Pillai, Kit Yeung, Yue Xiang Cheng, Tobias Cornvik, Par Nordlund, Megan Goh, Dilraj Lama, Zachary P. Gates, Chandra S. Verma, Dawn Thean, David P. Lane, Ignacio Asial, Christopher J. Brown
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-22 (2022)
Approaches have been devised to increase the discovery rate of intrabodies but often these yield results that aren’t functional in cells. Here the authors engineer and optimise an autonomous and disulphide-free human VH domain for intracellular exp
Externí odkaz:
https://doaj.org/article/e93e759ee68a4352930b901a4ebc4c2b
Autor:
Yuri Frosi, Shimin Jiang, Siti Radhiah Ramlan, Kelly Hew Hew, Alf Henrik Engman, Anil Pillai, Yue Xiang Cheng, Tobias Cornvik, Pär Nordlund, Megan Goh, Dilraj Lama, Chandra S Verma, Dawn Thean Thean, David P Lane, Ignacio Asial, Christopher Brown
An attractive approach to target intracellular macromolecular interfaces is to design small high affinity proteins. In this manuscript a stable, autonomous, human derived non-immunogenic, disulphide-free VH domain, has been engineered for intracellul
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5f80d30117a302deec2afff7f0e3fc6c
https://doi.org/10.21203/rs.3.rs-244469/v2
https://doi.org/10.21203/rs.3.rs-244469/v2
Autor:
Yuri Frosi, Simon Ng, Yen-Chu Lin, Shimin Jiang, Siti Radhiah Ramlan, Dilraj Lama, Chandra S. Verma, Ignacio Asial, Christopher J. Brown
Identifying new binding sites and poses that modify biological function are an important step towards drug discovery. We have identified a novel disulphide constrained peptide that interacts with the cap-binding site of eIF4E, an attractive therapeut
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::21f2ad63ad5176cc3611ece30b8a5eb4
https://doi.org/10.1101/2022.02.17.480295
https://doi.org/10.1101/2022.02.17.480295
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2025
Highly soluble and stable proteins are desirable for many different applications, from basic science to reaching a cancer patient in the form of a biological drug. For X-ray crystallography-where production of a protein crystal might take weeks and e
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::13bea0077beba569e825fd9e6fd16229
https://pubmed.ncbi.nlm.nih.gov/31267459
https://pubmed.ncbi.nlm.nih.gov/31267459
Publikováno v:
Methods in Molecular Biology ISBN: 9781493996230
Highly soluble and stable proteins are desirable for many different applications, from basic science to reaching a cancer patient in the form of a biological drug. For X-ray crystallography-where production of a protein crystal might take weeks and e
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3aff2332f73cd0e6306d7202dbeff6a6
https://doi.org/10.1007/978-1-4939-9624-7_14
https://doi.org/10.1007/978-1-4939-9624-7_14
Autor:
Ruchika Bajaj, Pascal F. Egea, Fei Li, Robert M. Stroud, Meghna Gupta, Alex J. Vecchio, Ignacio Asial, Brian C. Monk, Joana Paulino, Miles Sasha Dickinson, Shelagh Ferguson-Miller
Publikováno v:
Journal of Biological Chemistry. 296:100557
Biological membranes define the boundaries of cells and compartmentalize the chemical and physical processes required for life. Many biological processes are carried out by proteins embedded in or associated with such membranes. Determination of memb
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8f06d85ca396a63571173d514ff0dc79
https://doi.org/10.1016/j.jbc.2021.100557
https://doi.org/10.1016/j.jbc.2021.100557
Autor:
Tobias Cornvik, Yue Xiang Cheng, Henrik Engman, Pär Nordlund, Maria Dollhopf, Ignacio Asial, Binghuang Wu
Protein stability is often a limiting factor in the development of commercial proteins and biopharmaceuticals, as well as for biochemical and structural studies. Unfortunately, identifying stabilizing mutations is not trivial since most are neutral o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0a03363385ffeef135f92de955865675
http://hdl.handle.net/10220/19332
http://hdl.handle.net/10220/19332