Zobrazeno 1 - 10
of 239
pro vyhledávání: '"Ib Svendsen"'
Publikováno v:
Electrophoresis. 21:2660-2669
The proteomes of exponentially growing and stationary cells of Lactobacillus delbrueckii ssp. bulgaricus grown in rich medium (MRS) were separated by two-dimensional polyacrylamide gel electrophoresis (2-DE) and quantified after Coomassie staining. S
Publikováno v:
University of Copenhagen
Investigation of proteins extracted from wheat bran lead to the isolation of a 37 kDa polypeptide extracted from a polyacrylamide gel. Extensive internal peptide sequence information of this protein identified it as a glyoxalase I. Glyoxalase I activ
Publikováno v:
Journal of Plant Physiology. 156:468-476
Summary A protease was purified from green barley (Hordeum vulgare L.) malt with an apparent molecular mass of 74 kD and a pI of 6.9. Activity was assayed using an internally quenched fluorogenic peptide substrate, and the inhibitor profile indicated
Autor:
Karel Lemr, Osamu Yamauchi, Masaaki Endo, Pavel Peč, Ib Svendsen, Ivo Frébort, Marek Šebela, Shun Hirota, Andrea Bellelli
Publikováno v:
European Journal of Biochemistry. 267:1423-1433
2-Butyne-1,4-diamine (DABI) is a mechanism-based inhibitor of copper-containing plant amine oxidases; the number of turnovers that leads to enzyme inactivation is ≈ 20. The product of DABI oxidation is a very reactive aminoallene that reacts with a
Autor:
Karl Kristian Thomsen, Ib Svendsen, Anne Davy, David J. Simpson, Lira C Alves, Maria A. Juliano
Publikováno v:
Plant Physiology. 122:425-432
Barley (Hordeum vulgare L.) storage proteins, which have a high content of proline (Pro) and glutamine, are cleaved by cysteine endoproteases to yield peptides with a Pro next to the N-terminal and/or C-terminal amino acid residues. A peptidase cleav
Publikováno v:
Journal of Biological Chemistry. 275:1966-1975
The first committed steps in the biosynthesis of the two cyanogenic glucosides linamarin and lotaustralin in cassava are the conversion of l-valine andl-isoleucine, respectively, to the corresponding oximes. Two full-length cDNA clones that encode cy
Publikováno v:
Journal of Cereal Science. 30:237-244
Several different protease inhibitors have been identified in the mature barley grain, which are proposed to play a defensive role against potential barley pathogens. Cysteine protease inhibitors have been detected in mature grain and in the early st
Autor:
Morten Meldal, Mikael Blom Sørensen, Susanne Sørensen, Anne Davy, Verena Cameron-Mills, Ib Svendsen, David J. Simpson, Jacques Rouster
Publikováno v:
Plant Physiology. 117:255-261
The cysteine endoproteases (EP)-A and EP-B were purified from green barley (Hordeum vulgareL.) malt, and their identity was confirmed by N-terminal amino acid sequencing. EP-B cleavage sites in recombinant type-C hordein were determined by N-terminal
Autor:
Elaine Del Nery, Julio Scharfstein, Morten Meldal, Ib Svendsen, Maria A. Juliano, Luiz Juliano
Publikováno v:
Journal of Peptide Science. 4:83-91
A PEGA-resin was derivatized with a 3:1 mixture of hydroxymethyl benzoic acid and Fmoc-Lys(Boc)-OH and the fluorogenic substrate Ac-Y(NO2)KLRFSKQK(Abz)–PEGA was assembled on the lysine using the active ester approach. Following esterification of th
Autor:
Adam Szpacenko, Ib Svendsen, Monica M. Palcic, Christine H. Scaman, Andrew Holt, Glen R. Loppnow, Gordon Alton
Publikováno v:
Biochemistry. 37:4946-4957
Mammalian semicarbazide-sensitive amine oxidase (SSAO) enzymes have been classified as EC 1.4.3.6 [amine:oxygen oxidoreductase (deaminating)(copper-containing)]. However, both the identity of the quinone cofactor and the presence of copper remain unc