Zobrazeno 1 - 10
of 46
pro vyhledávání: '"Iain W. McNae"'
Autor:
Iain W. McNae, James Kinkead, Divya Malik, Li-Hsuan Yen, Martin K. Walker, Chris Swain, Scott P. Webster, Nick Gray, Peter M. Fernandes, Elmarie Myburgh, Elizabeth A. Blackburn, Ryan Ritchie, Carol Austin, Martin A. Wear, Adrian J. Highton, Andrew J. Keats, Antonio Vong, Jacqueline Dornan, Jeremy C. Mottram, Paul A. M. Michels, Simon Pettit, Malcolm D. Walkinshaw
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-10 (2021)
Glycolytic enzymes are challenging drug targets due to their highly conserved active sites and phosphorylated substrates. Here, the authors identify fast acting allosteric inhibitors of Trypanosoma brucei phosphofructokinase that block trypanosome gl
Externí odkaz:
https://doaj.org/article/4b5f2d939f554cc8b893fd1148232b4e
Autor:
Martin A. Wear, Matthew W. Nowicki, Elizabeth A. Blackburn, Iain W. McNae, Malcolm D. Walkinshaw
Publikováno v:
FEBS Open Bio, Vol 7, Iss 4, Pp 533-549 (2017)
We have established a refined methodology for generating surface plasmon resonance sensor surfaces of recombinant his‐tagged human cyclophilin‐A. Our orientation‐specific stabilisation approach captures his‐tagged protein under ‘physiologic
Externí odkaz:
https://doaj.org/article/2b897559d29a477eb4e1bf2d09d79cd3
Autor:
Hugh P. Morgan, Wenhe Zhong, Iain W. McNae, Paul A. M. Michels, Linda A. Fothergill-Gilmore, Malcolm D. Walkinshaw
Publikováno v:
Royal Society Open Science, Vol 1, Iss 1 (2014)
The transition between the inactive T-state (apoenzyme) and active R-state (effector bound enzyme) of Trypanosoma cruzi pyruvate kinase (PYK) is accompanied by a symmetrical 8° rigid body rocking motion of the A- and C-domain cores in each of the fo
Externí odkaz:
https://doaj.org/article/b7411e07cd9e47569f4972c21c4a6542
Publikováno v:
Biochemical Journal
6-Phosphofructokinase-1-kinase (PFK) tetramers catalyse the phosphorylation of fructose 6-phosphate (F6P) to fructose 1,6-bisphosphate (F16BP). Vertebrates have three PFK isoforms (PFK-M, PFK-L, and PFK-P). This study is the first to compare the kine
Autor:
James Kinkead, Malcolm D. Walkinshaw, Martin A. Wear, Monserrat Vásquez-Valdivieso, Iain W. McNae, Peter M Fernandes, Paul A.M. Michels
Publikováno v:
The Febs Journal
Fernandes, P, Kinkead, J, McNae, I, Vásquez-Valdivieso, M G, Wear, M, Michels, P & Walkinshaw, M 2019, ' Kinetic and structural studies of Trypanosoma and Leishmania phosphofructokinases show evolutionary divergence and identify AMP as a switch regulating glycolysis versus gluconeogenesis ', Febs Journal . https://doi.org/10.1111/febs.15177
Fernandes, P, Kinkead, J, McNae, I, Vásquez-Valdivieso, M G, Wear, M, Michels, P & Walkinshaw, M 2019, ' Kinetic and structural studies of Trypanosoma and Leishmania phosphofructokinases show evolutionary divergence and identify AMP as a switch regulating glycolysis versus gluconeogenesis ', Febs Journal . https://doi.org/10.1111/febs.15177
Trypanosomatids possess glycosome organelles that contain much of the glycolytic machinery, including phosphofructokinase (PFK). We present kinetic and structural data for PFK from three human pathogenic trypanosomatids, illustrating intriguing diffe
Autor:
James Kinkead, Paul A.M. Michels, Peter M Fernandes, Iain W. McNae, Frédéric Bringaud, Malcolm D. Walkinshaw
Publikováno v:
Fernandes, P M, Kinkead, J R, McNae, I W, Michels, P A & Walkinshaw, M D 2019, ' The kinetic characteristics of human and trypanosomatid phosphofructokinases for the reverse reaction ', Biochemical Journal, vol. 476, no. 2, pp. 179-191 . https://doi.org/10.1042/BCJ20180635
Biochemical Journal
Biochemical Journal, Portland Press, 2019, 476 (2), pp.179-191. ⟨10.1042/BCJ20180635⟩
Biochemical Journal
Biochemical Journal, Portland Press, 2019, 476 (2), pp.179-191. ⟨10.1042/BCJ20180635⟩
Eukaryotic ATP-dependent phosphofructokinases (PFKs) are often considered unidirectional enzymes catalysing the transfer of a phospho moiety from ATP to fructose 6-phosphate to produce ADP and fructose 1,6-bisphosphate. The reverse reaction is not ge
Autor:
James Kinkead, Ryan Ritchie, Paul A.M. Michels, Simon N. Pettit, Adrian J. Highton, Jeremy C. Mottram, Elizabeth A. Blackburn, Carol Austin, Martin Walker, Martin A. Wear, Antonio K. Vong, Malcolm D. Walkinshaw, Andrew John Keats, Scott P. Webster, Divya Malik, Nick Gray, Chris Swain, Li-Hsuan Yen, Iain W. McNae, Peter M Fernandes, Jacqueline Dornan, Elmarie Myburgh
Publikováno v:
McNae, I W, Kinkead, J, Malik, D, Yen, L-H, Walker, M K, Swain, C, Webster, S P, Gray, N, Fernandes, P M, Myburgh, E, Blackburn, E A, Ritchie, R, Austin, C, Wear, M A, Highton, A J, Keats, A J, Vong, A, Dornan, J, Mottram, J C, Michels, P A M, Pettit, S & Walkinshaw, M D 2021, ' Fast acting allosteric phosphofructokinase inhibitors block trypanosome glycolysis and cure acute African trypanosomiasis in mice ', Nature Communications, vol. 12, no. 1, 1052 . https://doi.org/10.1038/s41467-021-21273-6
Nature Communications
Nature Communications, Vol 12, Iss 1, Pp 1-10 (2021)
Nature Communications
Nature Communications, Vol 12, Iss 1, Pp 1-10 (2021)
The parasitic protist Trypanosoma brucei is the causative agent of Human African Trypanosomiasis, also known as sleeping sickness. The parasite enters the blood via the bite of the tsetse fly where it is wholly reliant on glycolysis for the productio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::799da05836121fe3900af19fdb8614ee
https://hdl.handle.net/20.500.11820/ba80df50-0eee-4cf5-92f8-bfbfbb7b3473
https://hdl.handle.net/20.500.11820/ba80df50-0eee-4cf5-92f8-bfbfbb7b3473
Autor:
Alice Rose, Mitchell, Meng, Yuan, Hugh P, Morgan, Iain W, McNae, Elizabeth A, Blackburn, Thierry, Le Bihan, Rafael A, Homem, Manda, Yu, Gary J, Loake, Paul A, Michels, Martin A, Wear, Malcolm D, Walkinshaw
Publikováno v:
Biochemical Journal
We show here that the M2 isoform of human pyruvate kinase (M2PYK) is susceptible to nitrosation and oxidation, and that these modifications regulate enzyme activity by preventing the formation of the active tetrameric form. The biotin-switch assay ca
Autor:
Harris Ioannidis, Julien Michel, Martin A. Wear, Malcolm D. Walkinshaw, Charis Georgiou, Iain W. McNae
Publikováno v:
Georgiou, C, Mcnae, I, Wear, M, Ioannidis, H, Michel, J & Walkinshaw, M 2017, ' Pushing the limits of detection of weak binding using fragment based drug discovery: identification of new cyclophilin binders ', Journal of Molecular Biology . https://doi.org/10.1016/j.jmb.2017.06.016
Fragment Based Drug Discovery(FBDD) isan increasingly popular method to identify novel small-molecule drug candidates. One of the limitations of the approach is the difficulty of accurately characterizing weak binding events. This work reports a comb
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9ab4e69e894d270ae11ceb5d10dda086
https://doi.org/10.1101/136101
https://doi.org/10.1101/136101
Autor:
Malcolm D. Walkinshaw, Elizabeth A. Blackburn, Matthew W. Nowicki, Martin A. Wear, Iain W. McNae
Publikováno v:
FEBS Open Bio
Wear, M, Nowicki, M, Blackburn, E, McNae, I & Walkinshaw, M 2017, ' Thermo-kinetic analysis space expansion for cyclophilin-ligand interactions; identification of a new non-peptide inhibitor using Biacore™ T200 ', FEBS Open Bio . https://doi.org/10.1002/2211-5463.12201
Wear, M, Nowicki, M, Blackburn, E, McNae, I & Walkinshaw, M 2017, ' Thermo-kinetic analysis space expansion for cyclophilin-ligand interactions; identification of a new non-peptide inhibitor using Biacore™ T200 ', FEBS Open Bio . https://doi.org/10.1002/2211-5463.12201
We have established a refined methodology for generating surface plasmon resonance sensor surfaces of recombinant his-tagged human cyclophilin-A. Our orientation-specific stabilisation approach captures his-tagged protein under “physiological condi