Zobrazeno 1 - 10
of 25
pro vyhledávání: '"IT Nisbet"'
Autor:
D. D. Shukla, IT Nisbet, M. B. Hayden, Mittur N. Jagadish, P Schoofs, S. J. Edwards, Colin W. Ward, Julian Grusovin, R.C. Hamilton, M McNamara, Kirsten Vandenberg, H Kalnins, J. A. Haynes, D. Pye
Publikováno v:
Intervirology. 39:85-92
Presentation of subunit vaccines in a highly ordered aggregate form can result in enhanced immune responses. Coat protein (CP) monomers of a potyvirus (Johnsongrass mosaic virus) when produced in heterologous host expression systems (Escherichia coli
Autor:
Dennis L. Stevens, Julian I. Rood, IT Nisbet, Milena M. Awad, Michael Lyristis, Joan Sloan, Amy E. Bryant
Publikováno v:
Molecular Microbiology. 12:761-777
Summary The anaerobic bacterium Clostridium perfringens mediates clostridial myonecrosis, or gas gangrene, by producing a number of extracellular toxins and enzymes. Transposon mutagenesis with Tn916 was used to isolate a pleiotropic mutant of C. per
Autor:
Mittur N. Jagadish, IT Nisbet, Caroline S. Fernandez, Harry Kalnins, R.C. Hamilton, Colin W. Ward, Peter Schoofs, K.M. Davern
Publikováno v:
Nature Biotechnology. 11:1166-1170
Synthesis in E. coli of native coat protein of Johnsongrass mosaic virus, and hybrid protein molecules containing foreign antigens, resulted in the intracellular formation of potyvirus-like particles (PVLPs). The foreign antigens used were an octapep
Autor:
Elizabeth Ann Webb, IT Nisbet
Publikováno v:
Biotechnology Letters. 15:889-894
Reinstating two naturally occurring intervening sequences of the human factor VIII gene into a B domain-deleted factor VIII cDNA sequence, either as separate entities or in combination, revealed slightly elevated protein production in the case of int
Publikováno v:
Biochemical and Biophysical Research Communications. 190:536-543
Factor VIII is a complex, plasma glycoprotein involved in the process of blood coagulation. Production of the recombinant molecule has largely been confined to mammalian cell systems which have, in general, proven to be inefficient producers of facto
Publikováno v:
Cytotechnology. 21(2)
The light and truncated heavy chains of human factor VIII, expressed separately in baculovirus-infected insect cells, exhibited different secretory behaviour when compared with each other and with a biologically active fusion molecule of the truncate
Publikováno v:
Archives of virology. 136(3-4)
The full length gene for the coat protein (CP) of the potyvirus, Johnsongrass mosaic virus, was incorporated into recombinant baculovirus and expressed in insect cells. Western blot and Coomassie-stained polyacrylamide gel electrophoresis analysis of
Publikováno v:
Gene. 119(1)
The gene pld, encoding the phospholipase D (PLD) of Corynebacterium pseudotuberculosis, was mutagenized using formic acid and then expressed in Escherichia coli. Mutagenesis was targeted at the coding region of pld, so as to produce only one or a lim
Publikováno v:
Nature Biotechnology. 15:731-732
A sound infrastructure for biotechnology creates opportunities for international investors.
Publikováno v:
Europe PubMed Central
The phospholipase D (PLD) gene from Corynebacterium pseudotuberculosis has been cloned, sequenced, and expressed in Escherichia coli. Analysis of DNA sequence data reveals a major open reading frame encoding a 31.4-kilodalton protein, a size consiste
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5d69e94567c08f696896b959ddf88250
http://europepmc.org/abstract/med/2407718
http://europepmc.org/abstract/med/2407718