Enhanced recombinant expression and purification of human IRAP for biochemical and crystallography studies

Autor: Hwai-Chen Guo, Lufei Sui

Publikováno v: Biochemistry and Biophysics Reports, Vol 27, Iss, Pp 101042-(2021)
Biochemistry and Biophysics Reports

Insulin-regulated aminopeptidase (IRAP) in humans is a membrane bound enzyme that has multiple functions. It was first described as a companion protein of the insulin-responsive glucose transporter, Glut4, in specialized vesicles. The protein has sub

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9f29b661aba2326d9e75ff81058b7497
http://www.sciencedirect.com/science/article/pii/S2405580821001369

Improved production of SARS-CoV-2 spike receptor-binding domain (RBD) for serology assays

Autor: Matthew Drew, Vanessa Wall, Kaitlyn Sadtler, Troy Taylor, Dominic Esposito, William K. Gillette, John-Paul Denson, Kelly Snead, Simon Messing, Jennifer Mehalko

Publikováno v: Protein Expression and Purification
bioRxiv
article-version (status) pre
article-version (number) 1

The receptor-binding domain (RBD) of the SARS-CoV-2 spike protein is a commonly used antigen for serology assays critical to determining the extent of SARS-CoV-2 exposure in the population. Different versions of the RBD protein have been developed an

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1cc806c227ce12e4cdbb0964da63baf2

The molecular basis of spectral tuning in blue- and red-shifted flavin-binding fluorescent proteins

Autor: Ulrich Krauss, Katrin Röllen, Kirill Kovalev, Joachim Granzin, Vera V. Nazarenko, Valentin Borshchevskiy, Valentin Gordeliy, Andrey Bogorodskiy, Ulrich Schwaneberg, Ivan Gushchin, Karl-Erich Jaeger, Alina Remeeva, Renu Batra-Safferling, Thomas Gensch, Stefanie Hemmer, Mehdi D. Davari

Publikováno v: The journal of biological chemistry 296, 100662 (2021). doi:10.1016/j.jbc.2021.100662
The Journal of Biological Chemistry
'Journal of Biological Chemistry ', vol: 296, pages: 100662-1-100662-13 (2021)
The journal of biological chemistry : JBC 296, 100662 (2021). doi:10.1016/j.jbc.2021.100662
The journal of biological chemistry 296, 100662-(2021). doi:10.1016/j.jbc.2021.100662

The journal of biological chemistry 296, 100662 (2021). doi:10.1016/j.jbc.2021.100662
Photoactive biological systems modify the optical properties of their chromophores, known as spectral tuning. Determining the molecular origin of spectral tuni

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::949f2c1d71af1df4c7a592ecf616b0c3

Structural insights into the mechanism of pH-selective substrate specificity of the polysaccharide lyase Smlt1473

Autor: Pranjal Mahanta, Bryan W. Berger, Rudresh Acharya, Shubhant Pandey

Publikováno v: The Journal of Biological Chemistry

Polysaccharide lyases (PLs) are a broad class of microbial enzymes that degrade anionic polysaccharides. Equally broad diversity in their polysaccharide substrates has attracted interest in biotechnological applications such as biomass conversion to

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2754ba648f190360c24a7ef5a1d04341
https://doi.org/10.1016/j.jbc.2021.101014

Structural and functional characterisation of ferret interleukin-2

Autor: Tam Pham, Karen L. Laurie, Zhenjun Chen, Timothy E. Adams, Daniel S. Layton, Andrew G. D. Bean, Kathryn Borg, Bin Ren, Janet Newman, Ian G. Barr, William J. McKinstry

Publikováno v: Developmental and Comparative Immunology

While the ferret is a valuable animal model for a number of human viral infections, such as influenza, Hendra and Nipah, evaluating the cellular immune response following infection has been hampered by the lack of a number of species-specific immunol

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e4a5dae4f51310c51aa4f625b65639ad
https://doi.org/10.1016/j.dci.2015.10.007