Zobrazeno 1 - 10
of 25
pro vyhledávání: '"I. M. Gerasymenko"'
Publikováno v:
Transgenic research. 31(3)
Cytochrome P450 monooxygenases (CYPs) are important tools for regio- and stereoselective oxidation of target molecules or engineering of metabolic pathways. Functional heterologous expression of eukaryotic CYPs is often problematic due to their depen
Engineering of a Plant Isoprenyl Diphosphate Synthase for Development of Irregular Coupling Activity
Autor:
Volker Schmidts, I. M. Gerasymenko, Ismael Navarro Fuertes, Lara Steinel, Yuriy V. Sheludko, Elisabeth Haumann, Heribert Warzecha
Publikováno v:
Chembiochem : a European journal of chemical biology. 23(1)
We performed mutagenesis on a regular isoprenyl diphosphate synthase (IDS), neryl diphosphate synthase from Solanum lycopersicum (SlNPPS), that has a structurally related analogue performing non-head-to-tail coupling of two dimethylallyl diphosphate
Autor:
Alfred Nordmann, Nicola J. Patron, Janine Gondolf, Kristina Gruden, Elena Moreno Giménez, Rubén Mateos Fernández, Marko Petek, Diego Orzaez, Špela Baebler, Kalyani Kallam, Mojca Juteršek, I. M. Gerasymenko
Publikováno v:
Plant Biotechnology Journal
Summary Arthropod crop pests are responsible for 20% of global annual crop losses, a figure predicted to increase in a changing climate where the ranges of numerous species are projected to expand. At the same time, many insect species are beneficial
Autor:
V. V. Kleschevnikov, L. O. Sakhno, I. A. Arbuzova, N. V. Kuchuk, I. M. Gerasymenko, Y. V. Sheludko, Victor E. Dosenko, V. R. Kedlian
Publikováno v:
Cytology and Genetics. 51:1-7
Development of RNAi-based therapeutics is a fast growing field of the pharmaceutical industry. Using plants for production of pharmaceutically valuable siRNAs may have significant advantages of costeffectiveness, scalability, and low risk of contamin
Biosynthetic capacity of plants, rooted in a near inexhaustible supply of photosynthetic energy and founded upon an intricate matrix of metabolic networks, makes them versatile chemists producing myriad specialized compounds. Along with tremendous su
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::70c964cd4034dc57de71618906ac53dd
https://doi.org/10.1016/bs.mie.2018.12.005
https://doi.org/10.1016/bs.mie.2018.12.005
Publikováno v:
Biotechnology journal. 13(11)
Employment of transient expression of foreign genes for bioconversion of pharmaceutically valuable low-molecular-weight compounds, including plant secondary metabolites, is an enticing trend still scantily explored in plant biotechnology. In the pres
Autor:
T. N. Kyrpa, I. V. Goldenkova-Pavlova, A. M. Ostapchuk, T. A. Hadjiev, Y. V. Sheludko, L. A. Sakhno, I. M. Gerasymenko
Publikováno v:
Russian Journal of Plant Physiology. 62:283-291
We established transgenic lines of Nicotiana tabacum expressing hybrid genes of Synechocystis sp. PCC 6803 Δ12 (desA) acyllipid desaturase and Synechococcus vulcanus Δ9 (desC) acyllipid desaturase with or without sequence coding for transit peptide
Autor:
Y. V. Sheludko, I. M. Gerasymenko
Publikováno v:
Biotechnology letters. 39(7)
To exploit cold-inducible biochemical processes beneficial for foreign mRNA transcription, translation and storage, as well as protein product stability, during Agrobacterium-mediated transient expression. The efficiency of three different 5′-regul
Autor:
V. A. Rudas, I. M. Gerasymenko, A. A. Klebanovych, T. M. Klein, Y. V. Sheludko, N. V. Kuchuk, A. M. Shakhovsky
Publikováno v:
Transgenic research. 26(1)
The development of tools which ensure the desired level of transgene expression in plastids is a prerequisite for the effective utilization of these plant organelles for the deployment of bioactive proteins. High-level accumulation of target proteins
Publikováno v:
Biopolymers and Cell, Vol 28, Iss 5, Pp 368-373 (2012)
Our aim was to investigate the influence of some exogenous agents on the recombinant protein accumulation in plants via Agrobacterium-mediated transient expression. Methods. Agrobacterium-mediated transient expression method, spectrophotometric metho