Virus-Induced Gene Silencing (VIGS) as a Reverse Genetic Tool to Study Development of Symbiotic Root Nodules

Autor: G. D. Constantin, M. Grønlund, I. E. Johansen, J. Stougaard, O. S. Lund

Publikováno v: Molecular Plant-Microbe Interactions, Vol 21, Iss 6, Pp 720-727 (2008)

Virus-induced gene silencing (VIGS) can provide a shortcut to plants with altered expression of specific genes. Here, we report that VIGS of the Nodule inception gene (Nin) can alter the nodulation phenotype and Nin gene expression in Pisum sativum.

Externí odkaz: https://doaj.org/article/63bfe82785ef426496520c5ca985e607

Potyvirus Genome-Linked Protein (VPg) Determines Pea Seed-Borne Mosaic Virus Pathotype-Specific Virulence in Pisum sativum

Autor: R. O. Hampton, Robert R. Martin, K. E. Keller, I. E. Johansen

Publikováno v: Molecular Plant-Microbe Interactions®. 11:124-130

The mechanism of Pisum sativum pathotype-specific resistance to pea seed-borne mosaic potyvirus (PSbMV) was investigated and the coding region determinant of PSbMV virulence was defined. Homozygous recessive sbm-1 peas are unable to support replicati

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ce19c6ea3f153df56ff516bd650393a0
https://doi.org/10.1094/mpmi.1998.11.2.124

Biological and molecular properties of a pathotype P-1 and a pathotype P-4 isolate of pea seed-borne mosaic virus

Autor: W. G. Dougherty, K. E. Keller, R. O. Hampton, I. E. Johansen

Publikováno v: Johansen, I E, Keller, K E, Dougherty, W G & Hampton, R O 1996, ' Biological and molecular properties of a pathotype P-1 and a pathotype P-4 isolate of pea seed-borne mosaic virus ' Journal of General Virology, vol. 77, pp. 1329-1333 .

Two isolates of pea seed-borne mosaic potyvirus, DPD1 and NY, were identified as pathotypes P-1 and P-4, respectively, by their infectivity on Pisum sativum L. lines homozygous for the recessive resistance genes sbm-1 and sbm-4. The two isolates diff

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0d3890ba43a80f40c3cd187694e6036d
https://doi.org/10.1099/0022-1317-77-6-1329

Specificity of resistance to pea seed-borne mosaic potyvirus in transgenic peas expressing the viral replicase (Nlb) gene

Autor: I Bach, I E Johansen, A L Jones, A J Maule, S J Bean

Publikováno v: The Journal of general virology. 79

Transgenic pea lines carrying the replicase (NIb) gene of pea seed-borne mosaic potyvirus (PSbMV) were generated and used in experiments to determine the effectiveness of induced resistance upon heterologous isolates. Three pea lines showed inducible

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d2be94a3283dd7da651e46c1a2147c2d
https://pubmed.ncbi.nlm.nih.gov/9880032

Characterization and molecular detection of the P4 pathotype of pea seedborne mosaic potyvirus

Autor: P. D. Kohnen, I. E. Johansen, R. O. Hampton

Publikováno v: Kohnen, P D, Johansen, I E & Hampton, R O 1995, ' Characterization and molecular detection of the P4 pathotype of pea seedborne mosaic potyvirus ', Phytopathology, vol. 85, pp. 789-793 .

The New York isolate of pea seedborne mosaic potyvirus (PSbMV) pathotype P4 was differentiated from the more prevalent PSbMV-P1 pathotype on selected pea cultivars, serologically and by sequence-specific reverse transcription-polymerase chain reactio

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fd602709692598325d4f911a27989510
https://pure.au.dk/portal/da/publications/characterization-and-molecular-detection-of-the-p4-pathotype-of-pea-seedborne-mosaic-potyvirus(6de14ff0-125e-11db-aa3e-000ea68e967b).html