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Agonist activation of p42 and p44 mitogen-activated protein kinases following expression of the mouse δ opioid receptor in Rat-1 fibroblasts: effects of receptor expression levels and comparisons with G-protein activation

Autor: Graeme Milligan, Andrew R. Burt, Ian Mullaney, Neil G. Anderson, I. C. Carr

Publikováno v: Biochemical Journal. 320:227-235

Rat-1 fibroblasts were transfected with a cDNA encoding the mouse Δ opioid receptor. Two separate clones, D2 (which expressed some 6 pmol of the receptor/mg of membrane protein) and DOE (which expressed some 0.2 pmol/mg of membrane protein), were ex

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4f292e9337fb92a8759cf3e710cb3197
https://doi.org/10.1042/bj3200227

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Analysis of inverse agonism at the δ opioid receptor after expression in Rat 1 fibroblasts

Autor: Ian Mullaney, Graeme Milligan, I. C. Carr

Publikováno v: Biochemical Journal. 315:227-234

A cDNA encoding the mouse delta opioid receptor was expressed stably in a Rat 1 fibroblast cell line. Expression of this receptor was demonstrated both in ligand binding studies and by reverse transcriptase-PCR. In membranes of clone D2 cells the opi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0c239a852c757c366cf0ee699e1167a1
https://doi.org/10.1042/bj3150227

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The long isoform of the rat thyrotropin-releasing hormone receptor down-regulates Gq proteins

Autor: Graeme Milligan, K. A. Eidne, J. Zabavnik, L. Anderson, Gun-Do Kim, I. C. Carr

Publikováno v: Journal of Biological Chemistry. 269:19933-19940

A cDNA encoding the long isoform of the rat thyrotropin releasing hormone (TRH) receptor was expressed stably in HEK-293 cells. Polymerase chain reaction analysis confirmed expression of mRNA encoding only the long and not the short isoform. Activati

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::d1edd4d83c1f4fc403a90dbdb72b7fb4
https://doi.org/10.1016/s0021-9258(17)32110-5

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Alpha 2-C10 adrenergic receptors expressed in rat 1 fibroblasts can regulate both adenylylcyclase and phospholipase D-mediated hydrolysis of phosphatidylcholine by interacting with pertussis toxin-sensitive guanine nucleotide-binding proteins

Autor: Elisabeth E. Macnulty, I. C. Carr, Steven J. McClue, Graeme Milligan, Michael J.O. Wakelam, Thomas J. Jess

Publikováno v: Journal of Biological Chemistry. 267:2149-2156

The alpha 2-C10 adrenergic receptor from human platelets was expressed permanently in Rat-1 fibroblasts. A series of clones that varied in expression of the receptor from 0 to 3.5 pmol/mg of membrane protein were isolated. We have demonstrated recent

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::020da6c524d1ed13c73d535e27736cc4
https://doi.org/10.1016/s0021-9258(18)45856-5

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Regulation of G protein activation and effector modulation by fusion proteins between the human 5-hydroxytryptamine(1A) receptor and the alpha subunit of G(i1): differences in receptor-constitutive activity imparted by single amino acid substitutions in G(i1)alpha

Autor: E, Kellett, I C, Carr, G, Milligan

Publikováno v: Molecular pharmacology. 56(4)

Fusion proteins were generated between the human 5-hydroxytryptamine (5-HT)(1A) receptor and both wild-type (Cys(351)) and pertussis toxin-resistant (Gly(351) and Ile(351)) forms of G(i1). These were expressed stably. Pertussis toxin treatment substa

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::47ea7d26265d8c4eebc78052fab51acf
https://pubmed.ncbi.nlm.nih.gov/10496950

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Measurement of agonist-induced guanine nucleotide turnover by the G-protein Gi1alpha when constrained within an alpha2A-adrenoceptor-Gi1alpha fusion protein

Autor: Alan Wise, Graeme Milligan, I. C. Carr

A fusion protein was generated between the porcine alpha2A-adrenoceptor and a pertussis-toxin-insensitive (Cys351-->Gly) variant of the alpha subunit of Gi1alpha by direct in-frame fusion of the N-terminus of the G-protein to the C-terminus of the re

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::04e5fbb6d1bfd16f40a30be55beab7ed
https://europepmc.org/articles/PMC1218523/

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Detection and analysis of agonist-induced formation of the complex of the stimulatory guanine nucleotide-binding protein with adenylate cyclase in intact wild-type and beta 2-adrenoceptor-expressing NG108-15 cells

Autor: Gun-Do Kim, Graeme Milligan, I. C. Carr

Neuroblastoma x glioma hybrid, NG108-15, cells appear to express the alpha-subunit of the guanine nucleotide-binding protein Gs in a substantial molar excess over its effector adenylate cyclase [Kim, Adie and Milligan (1994) Eur. J. Biochem. 219, 135

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::271f032d90388289a89257894e6d1286
https://europepmc.org/articles/PMC1136873/

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The long isoform of the rat thyrotropin-releasing hormone receptor down-regulates Gq proteins

Autor: G D, Kim, I C, Carr, L A, Anderson, J, Zabavnik, K A, Eidne, G, Milligan

Publikováno v: The Journal of biological chemistry. 269(31)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::9aa42872debd7cffa51dde00ba794ae7
https://pubmed.ncbi.nlm.nih.gov/8051077

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Autor: E E, MacNulty, S J, McClue, I C, Carr, T, Jess, M J, Wakelam, G, Milligan

Publikováno v: The Journal of biological chemistry. 267(4)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::d8e476f9bea8856c9d23871a13f085d8
https://pubmed.ncbi.nlm.nih.gov/1346392

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