Zobrazeno 1 - 10
of 107
pro vyhledávání: '"I. B., Holland"'
Publikováno v:
Mathematical Modelling of Natural Phenomena. 5:148-162
Bacillus subtilis swarms rapidly over the surface of a synthetic medium creating remarkable hyperbranched dendritic communities. Models to reproduce such effects have been proposed under the form of parabolic Partial Differential Equations representi
Publikováno v:
Infection & Immunity, 71(1), 163-172. American Society for Microbiology
Knockout mutations were constructed in the arcA gene of a virulent type b strain of Haemophilus influenzae , and the behavior of the resulting mutants was investigated in a number of conditions that mimicked distinct steps in the natural infection pa
Publikováno v:
Cell Calcium. 32:183-192
As in eukaryotes, bacterial free Ca(2+) can play an important role as an intracellular signal. However, because free Ca(2+) is difficult to measure in live bacteria, most of the evidence for such a role is indirect. Gram-negative bacteria also have a
Autor:
Young J, I. B. Holland
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Biomembranes. 1461(2):177-200
Publikováno v:
Biochimie. 81:901-907
We have previously proposed that fluctuations in Ca(2+) levels should play an important role in bacteria as in eukaryotes in regulating cell cycle events (Norris et al., J. Theor. Biol. 134 (1998) 341-350). This proposal implied the presence of Ca(2+
Publikováno v:
Molecular and General Genetics MGG. 261:122-132
HlyD has a single transmembrane domain (residues 59-80) and a large periplasmic domain, and is essential for the secretion of haemolysin from Escherichia coli. Using an antibody raised against HlyD, the protein was localised to the cell envelope by i
Publikováno v:
Molecular and General Genetics MGG. 259:645-655
In earlier studies we found that E. coli is sensitive to anticalmodulin drugs such as W7. Mutants that are resistant to this drug were isolated, including wseA1. In an attempt to clone the wseA gene, we isolated a clone that restored sensitivity to t
Publikováno v:
Molecular Microbiology. 20:1273-1286
Summary We describe a novel Escherichia coli protein, DjlA, containing a highly conserved J-region motif, which is present in the DnaJ protein chaperone family and required for interaction with DnaK. Remarkably, DjlA is shown to be a membrane protein
Autor:
I. B. Holland, C Chervaux
Publikováno v:
Journal of Bacteriology. 178:1232-1236
The HlyA secretion signal sequence of approximately 46 residues is predicted to contain helix I and an amphipathic helix II separated by a short loop including the conserved Phe residue, F-989. All nine substitutions of Phe-989 drastically reduce sec
Autor:
J. C. Lazzaroni, Mark A. Blight, A. L. Pimenta, L. Kotelevets, S. J. Séror, C. Dando, I. B. Holland
Publikováno v:
Molecular and General Genetics MGG. 245:431-440
We have carried out a genetic analysis of Escherichia coli HlyB using in vitro(hydroxylamine) mutagenesis and regionally directed mutagenesis. From random mutagenesis, three mutants, temperature sensitive (Ts) for secretion, were isolated and the DNA