Zobrazeno 1 - 10
of 588
pro vyhledávání: '"I. B., Holland"'
Publikováno v:
Journal of Molecular Biology. 317:765-788
The alpha-mating pheromone receptor encoded by the yeast STE2 gene is a G protein coupled receptor that initiates signaling via a MAP kinase pathway that prepares haploid cells for mating. To establish the range of allowed amino acid substitutions wi
Autor:
Liesbeth M. Veenhoff, Bert Poolman, Esther H.M.L. Heuberger, Robert H.E. Friesen, Ria H. Duurkens
Publikováno v:
Journal of Molecular Biology. 317:591-600
Blue native electrophoresis is used widely for the analysis of non-dissociated protein complexes with respect to composition, oligomeric state and molecular mass. However, the effects of detergent or dye binding on the mass and stability of the integ
Publikováno v:
Journal of Molecular Biology. 315:799-807
alpha-Synuclein (alpha-Syn) is an abundant presynaptic protein of unknown function, which has been implicated in the pathogenesis of Parkinson's disease. Alpha-Syn has been suggested to play a role in lipid transport and synaptogenesis, and growing e
Publikováno v:
Journal of Molecular Biology. 315:831-843
Preprotein translocase is a general and essential system for bacterial protein export, the minimal components of which are SecA and SecYEG. SecA is a peripheral ATPase that associates with nucleotide, preprotein, and the membrane integral SecYEG to f
Publikováno v:
Journal of Molecular Biology. 315:53-62
By using functional lactose permease devoid of native Cys residues with a discontinuity in the periplasmic loop between helices VII and VIII (N7/C5 split permease), cross-linking between engineered paired Cys residues in helices VII and X was studied
Publikováno v:
Journal of Molecular Biology. 314:709-716
Length polymorphism due to tandem repeats is a common feature in animal mitochondrial DNA. The rabbit mitochondrial genome contains a 20 bp repeat domain, which generates a general heteroplasmic state. The observed polymorphic patterns suggest a dyna
Publikováno v:
Journal of Molecular Biology. 314:375-386
Transcription attenuation comprises several processes that affect transcript elongation and transcription termination, and has an important role in regulating gene expression. In most cases, transcription attenuation is used as a regulatory mechanism
Publikováno v:
Journal of Molecular Biology. 312:899-906
The prophage of coliphage N15 is not integrated into the bacterial chromosome but exists as a linear plasmid molecule with covalently closed ends. Upon infection of an Escherichia coli cell, the phage DNA circularises via cohesive ends. A phage-encod
Autor:
Mirjam Czjzek, Michel Juy, Frédéric Barras, Mohammed El Hassouni, Béatrice Py, Virginie Chapon
Publikováno v:
Journal of Molecular Biology. 310:1055-1066
Erwinia chrysanthemi, a Gram-negative plant pathogen, secretes the cellulase Cel5 (formerly EGZ) via the type II secretion pathway (referred to as Out). Cel5 is composed of two domains, a large N-terminal catalytic domain (390 amino acid residues) an
Publikováno v:
Journal of Molecular Biology. 308:221-229
Assembly of the long helical filament of the bacterial flagellum requires polymerisation of ca 20,000 flagellin (FliC) monomeric subunits into the growing structure extending from the cell surface. Here, we show that export of Salmonella flagellin is