Zobrazeno 1 - 10
of 18
pro vyhledávání: '"I. A. Ryabukhin"'
Autor:
I. A. Ryabukhin, Yu. A. Zhirkov, I. A. Kashparov, Vladimir P. Chekhonin, T. B. Dmitriyeva, Olga I. Gurina, S. V. Lebedev
Publikováno v:
Drug Delivery. 12:1-6
Polyethylene glycol (PEG)ylated (stealth) immunoliposomes directed against human gliofibrillary acidic protein (GFAP) were prepared by coupling the thiolated monoclonal anti-GFAP antibodies with a maleimide derivative of phosphatidyl ethanolamine of
Autor:
Dmitrieva Tb, Yu. A. Zhirkov, Olga I. Gurina, Vladimir P. Chekhonin, S. V. Lebedev, D. V. Blinov, I. A. Ryabukhin, I. A. Kashparov
Publikováno v:
Doklady Biochemistry and Biophysics. 391:236-239
Autor:
I I Shepeleva, I. A. Ryabukhin, G N Babich, Olga I. Gurina, Vladimir P. Chekhonin, Dmitrieva Tb
Publikováno v:
Bulletin of Experimental Biology and Medicine. 132:772-775
Methods of GFAP purification and obtaining of hybridoma cells producing monoclonal anti-GFAP antibodies and properties of GFAP preparation were described. The immunobloting data on specificity of obtained monoclonal antibodies are presented. A new me
Publikováno v:
NeuroReport. 7:129-132
Publikováno v:
NeuroReport. 7:129-132
Hydrophobized and non-hydrophobized Fab fragments of human antibodies against gliofibrillar acid protein (GFAP) and brain specific alpha 2-glycoprotein (alpha 2GP) were used to study their penetration through the blood-brain barrier (BBB). These Fab
Autor:
Vladimir P. Baklaushev, I. A. Ryabukhin, N. E. Volgina, N. V. Ivanova, N. F. Grinenko, A. V. Leopol’d, Vladimir P. Chekhonin, A. A. Korchagina, S. A. Shein, Olga I. Gurina
Publikováno v:
Bulletin of experimental biology and medicine. 153(1)
Female BALB/c mice were subcutaneously immunized with recombinant VEGF-164. After 3 immunization cycles, splenic B cells from immunized mouse were fused with immortalized myeloma culture SP2/0-Ag14 cells. Screening of hybrid cells producing anti-VEGF
Autor:
Sergey A. Shein, N. F. Grinenko, A. V. Leopol’d, Vladimir P. Chekhonin, K. A. Pavlov, I. A. Ryabukhin, Vladimir P. Baklaushev, Anna A. Korchagina
Publikováno v:
Bulletin of experimental biology and medicine. 152(6)
cDNA encoding VEGF and Ig-like extracellular domains 2-4 of VEGFR-1 (sFlt-12-4) were cloned into prokaryotic expression vectors pET32a and pQE60. Recombinant proteins were purifi ed (metal affi nity chromatography) and renatured. Chemiluminescent stu
Autor:
Vladimir P. Baklaushev, A. V. Leopol’d, Vladimir P. Chekhonin, A. V. Semenova, Olga I. Gurina, K. Sh. Kardashova, I. A. Ryabukhin
Publikováno v:
Bulletin of experimental biology and medicine. 152(6)
Brain-specifi c anion transporter BSAT1 extracellular fragment (451-557) cDNA was cloned in a vector for prokaryotic expression, a producer E. coli strain was obtained, and recombinant extracellular fragment BSAT1 451-557 was purifi ed and used for i
Publikováno v:
Bulletin of Experimental Biology and Medicine. 131:502-504
Enzyme immunoassay showed penetration of two glia-specific antigens, glial fibrillar acid protein GFAP and specific brain glycoprotein α2GP, through the blood-brain barrier in rats treated with toxic doses of sodium barbital. The permeability of the
Autor:
Petrov Sv, I. A. Kashparov, Dmitrieva Tb, S. V. Lebedev, Olga I. Gurina, Vladimir P. Chekhonin, A. I. Volkov, I. A. Ryabukhin, Yu. S. Skoblov
Publikováno v:
Bulletin of experimental biology and medicine. 138(4)
Preparations of I(125)-labeled monoclonal antibodies against neurospecific enolase and mouse plasma IgG1 were injected intravenously to rats immediately after unilateral occlusion of the middle cerebral artery. Radioactivity of I(125)-labeled monoclo