Zobrazeno 1 - 10
of 36
pro vyhledávání: '"I M Reardon"'
Publikováno v:
Proteins: Structure, Function, and Genetics. 24:51-72
Based on the sequence-coupled (Markov chain) model and vector-projection principle, a discriminant function method is proposed to predict sites in protein substrates that should be susceptible to cleavage by the HIV-1 protease. The discriminant funct
Autor:
D J Rothrock, Carol A. Bannow, C S Tomich, J M Lull, A. M. Mildner, I M Reardon, J L Sarcich, Clark W. Smith, W J Howe, Joseph W. Leone
Publikováno v:
Biochemistry. 33:9405-9413
Site-directed mutagenesis of autolysis sites in the human immunodeficiency virus type 1 (HIV-1) protease was applied in an analysis of enzyme specificity; the protease served, therefore, as both enzyme and substrate in this study. Inspection of natur
Publikováno v:
Biochemistry. 32:12571-12576
We have previously identified hsp56, a protein component of steroid receptor complexes, as an FK506 binding protein [Yem et al. (1992) J. Biol. Chem. 267, 2868-2871]. We now report that hsp56 is also found to be a major immunophilin in chicken thymus
Autor:
A G Tomasselli, J. O. Hui, Robert L. Heinrikson, Che-Shen C. Tomich, June Lull, David P. Brunner, I M Reardon
Publikováno v:
Journal of Protein Chemistry. 12:323-327
The protease encoded by the human immunodeficiency virus type 1 (HIV-1) was engineered in Escherichia coli as a construct in which the natural 99-residue polypeptide was preceded by an NH2-terminal methionine initiator. Inclusion bodies harboring the
Autor:
R L Heinrikson, J D Lowenson, I M Reardon, H A Zürcher-Neely, R J Cotter, Rong Wang, Melvyn J. Ball, Steven Clarke, C Wolkow, Alex E. Roher
Publikováno v:
Journal of Biological Chemistry. 268:3072-3083
The structure of beta-amyloid (beta A) from Alzheimer disease brains was examined to determine if post-translational modifications might be linked to the abnormal deposition of this peptide in the diseased tissue. The beta A peptides were isolated fr
Autor:
P L Munns, I M Reardon, Anthony W. Yem, V A Ruff, Martin R. Deibel, L D Adams, Karen L. Leach
Publikováno v:
Journal of Biological Chemistry. 267:21285-21288
Heat shock protein 56 (hsp56) has been shown to be involved in two cellular pathways, as an immunophilin for FK506 and as a component of steroid receptor complexes. To help define its role in these cellular pathways, we have developed UPJ56, a polycl
Autor:
Carol A. Bannow, J. O. Hui, Clark W. Smith, H A Zurcher-Neely, I M Reardon, Martin R. Deibel, A G Tomasselli, Robert L. Heinrikson
Publikováno v:
Journal of Biological Chemistry. 267:10232-10237
The protease from simian immunodeficiency virus (SIV) was chemically synthesized by automated solid-phase technology as an NH2-terminally extended derivative, capped with biotin. Biotin-linker-(SIV protease (1-99)): the linker segment, Gly-Gly-Asp-Ar
Publikováno v:
Journal of Virology. 65:5440-5447
The sequence of the 3'-terminal 2,486 bases of the feline calicivirus (FCV) genome was determined. This region of the FCV genome, from which the 2.4-kb subgenomic RNA is derived, contained two open reading frames. The larger open reading frame, found
Autor:
Scott E. Truesdell, Robert L. Heinrikson, I M Reardon, Michael J. Bienkowski, Alice L. Laborde, J. A. Shelly, Ann E. Berger, J G Chosay, Thomas E. Eessalu, H A Zurcher-Neely
Publikováno v:
Journal of Biological Chemistry. 265:14505-14511
Culture medium conditioned by phorbol 12-myristate 13-acetate-differentiated THP-1 cells contained interleukin 1 (IL-1) antagonist activity as measured by inhibition of both IL-1 beta binding to receptors on YT cells and inhibition of IL-1/phytohemag
Autor:
W. J. Howe, Robert L. Heinrikson, Carol A. Bannow, J. O. Hui, A G Tomasselli, D L DeCamp, I M Reardon, C S Craik, D J Staples, Tomi K. Sawyer
Publikováno v:
Journal of Biological Chemistry. 265:14675-14683
Highly purified, recombinant preparations of the virally encoded proteases from human immunodeficiency viruses (HIV) 1 and 2 have been compared relative to 1) their specificities toward non-viral protein and synthetic peptide substrates, and 2) their