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Highly sensitive northern hybridization using a rapid protocol for downward alkaline blotting of RNA
Publikováno v:
BioTechniques. 25(3)
A simple and fast RNA gel blot procedure is described that uses 50 mM NaOH to simultaneously transfer and fix RNA to a positively charged nylon membrane. The RNA is transferred in a downward direction, and transfer is routinely completed within 2.5 h