Zobrazeno 1 - 10
of 32
pro vyhledávání: '"I B, Leshchinskaya"'
Autor:
Nelly P. Balaban, Ilya V. Demidyuk, Airat R. Kayumov, Margarita R. Sharipova, T.V. Akimkina, Yuliya Kirillova, D. R. Safina, Galina N. Rudenskaya, Sergey V. Kostrov, I. B. Leshchinskaya, L. A. Gabdrakhmanova, Ayslu Mardanova
Publikováno v:
Microbiological Research. 163(1):39-50
The gene encoding for Bacillus intermedius serine proteinase was cloned and the complete nucleotide sequence was determined. Gene expression was explored in the protease-deficient strain Bacillus subtilis AJ73 during different stages of growth. Catab
Autor:
L. A. Gabdrakhmanova, Nelly P. Balaban, Sergey V. Kostrov, I. B. Leshchinskaya, Innokentii Vishniakov, Margarita R. Sharipova
Publikováno v:
Microbiological Research. 160(3):233-242
Summary Bacteria from the genus Bacillus have evolved complicated regulatory networks to be protected from various environmental stresses, including sudden increase in salinity. Among these regulatory mechanisms is the DegS–DegU signal transduction
Autor:
Margarita R. Sharipova, Sergey V. Kostrov, Galina N. Rudenskaya, Nelly P. Balaban, L. A. Gabdrakhmanova, I. B. Leshchinskaya, Inna B. Chastukhina
Publikováno v:
Microbiology. 74:32-39
We studied the biosynthesis of bacillus intermedius glutamyl endopeptidase in the recombinant bacillus subtilis strain AJ73 Δ58.21 during the stationary growth phase. We optimized the composition of the culture medium to favor effective enzyme produ
Autor:
I. B. Leshchinskaya, Margarita R. Sharipova, E. A. Sokolova, Nelly P. Balaban, Ayslu Mardanova, L. A. Gabdrakhmanova, Galina N. Rudenskaya
Publikováno v:
Biochemistry (Moscow). 69:420-426
A proteinase secreted in the late stationary phase was isolated from the culture fluid of Bacillus intermedius 3-19 by ion-exchange chromatography on CM-cellulose followed by FPLC on a Mono S column. The enzyme was completely inhibited by the serine
Autor:
Ayslu Mardanova, Nelly P. Balaban, Margarita R. Sharipova, E. A. Sokolova, L. A. Gabdrakhmanova, Galina N. Rudenskaya, I. B. Leshchinskaya, Yu. S. Tokmakova
Publikováno v:
Microbiology. 72:295-299
In the late stages of sporulation, cells of Bacillus intermedius 3-19 secreted into the medium two proteinases, glutamyl endopeptidase and subtilisin, whose maximum activities were recorded in the 40th and 44th hours of growth, respectively. By estim
Autor:
Nelly P. Balaban, I. B. Leshchinskaya, Galina N. Rudenskaya, E. A. Sokolova, Yu. S. Tokmakova, Margarita R. Sharipova, L. A. Gabdrakhmanova
Publikováno v:
Microbiology. 71:275-280
The effect of some components of cultivation medium on the growth of the streptomycin-resistant Bacillus intermedius strain 3-19 and on the production of glutamyl endopeptidase was investigated using factorial experimental design, which allowed the c
Autor:
Nelly P. Balaban, Galina N. Rudenskaya, I. B. Leshchinskaya, L. A. Gabdrakhmanova, Shilova Ma, Margarita R. Sharipova, Yu. M. Kadyrova
Publikováno v:
Microbiology. 71:420-424
The investigation of the activity of extracellular hydrolytic enzymes and sporulation in the bacterium Bacillus intermedius 3-19 showed that the activity of ribonuclease is maximal in the glucose-containing growth medium, in which sporulation is supp
Autor:
N. A. Cherepanova, Valentina P. Gubskaya, I. B. Leshchinskaya, Michael J. Benedik, I. A. Nuretdinov, M. N. Filimonova
Publikováno v:
Biochemistry (Moscow). 66:323-327
The mechanism of action of p-chloromercuribenzoate (PCMB) on Serratia marcescens nuclease was investigated. The analysis showed that PCMB forms complexes with DNA. Binding of C7H5O2Hg+ to DNA changes the secondary structure of the DNA. These changes
Autor:
L. A. Gabdrakhmanova, Eugene V. Shakirov, Shilova Ma, Nelly P. Balaban, Margarita R. Sharipova, Galina N. Rudenskaya, I. B. Leshchinskaya
Publikováno v:
Microbiology. 69:552-558
The activities of proteinases in the culture fluid and cellular fractions ofBacillus intermedius 3–19 grown under various conditions were studied. Thiol-dependent serine proteinase was the prevalent enzyme in the total pool of extracellular protein
Autor:
I. B. Leshchinskaya, L. A. Gabdrakhmanova, Sergey V. Kostrov, Galina N. Rudenskaya, Nelly P. Balaban, Margarita R. Sharipova, T.V. Akimkina, Eugene V. Shakirov
Publikováno v:
Microbiology. 69:546-551
A nutrient medium was elaborated for the efficient production of glutamyl endopeptidase by the recombinantBacillus subtilis strain AJ73 bearing theBacillus intermedius 3–19 glutamyl endopeptidase gene within a multicopy plasmid. Optimal concentrati