Zobrazeno 1 - 10
of 59
pro vyhledávání: '"I B, Leshchinskaia"'
Autor:
I B, Chastukhina, M R, Sharipova, L A, Gabdrakhmanova, N P, Balaban, S V, Kostrov, G N, Rudenskaia, I B, Leshchinskaia
Publikováno v:
Mikrobiologiia. 74(1)
We studied the biosynthesis of Bacillus intermedius glutamyl endopeptidase in the recombinant Bacillus subtilis strain AJ73 delta58.21 during the stationary growth phase. We optimized the composition of the culture medium to favor effective enzyme pr
Publikováno v:
Mikrobiologiia. 74(1)
The investigation of the effect of some components of the medium on the distribution of the secretory guanyl-specific ribonuclease of Bacillus intermedius (EC 3.1.4.23) among various cell fractions and culture liquid showed that the amount of this en
Autor:
I B, Chastukhina, M P, Sharipova, L A, Gabdrakhmanova, N P, Balaban, D R, Safina, S V, Kostrov, G N, Rudenskaia, I B, Leshchinskaia
Publikováno v:
Mikrobiologiia. 73(3)
The growth of the recombinant Bacillus subtilis strain AJ73 carrying the Bacillus intermedius 3-19 glutamyl endopeptidase gene on a multicopy plasmid and the effect of some nutrients on the efficiency of extracellular glutamyl endopeptidase productio
Autor:
N P, Balaban, M P, Sharipova, L A, Gabdrakhmanova, A M, Mardanova, Iu S, Tokmakova, E A, Sokolova, G N, Rudenskaia, I B, Leshchinskaia
Publikováno v:
Mikrobiologiia. 72(3)
In the late stages of sporulation, cells of Bacillus intermedius 3-19 secreted into the medium two proteinases, glutamyl endopeptidase and subtilisin, whose maximum activities were recorded in the 40th and 44th hours of growth, respectively. By estim
Autor:
M R, Sharipova, N P, Balaban, L A, Gabdrakhmanova, M A, Shilova, Iu M, Kadyrova, G N, Rudenskaia, I B, Leshchinskaia
Publikováno v:
Mikrobiologiia. 71(4)
The investigation of the activity of extracellular hydrolytic enzymes and sporulation in the bacterium Bacillus intermedius 3-19 showed that the activity of ribonuclease is maximal in the glucose-containing growth medium, in which sporulation is supp
Publikováno v:
Molekuliarnaia genetika, mikrobiologiia i virusologiia. (2)
Under phosphate-deficient conditions, B. intermedius, B. pumilus, and B. thuringiensis secrete phosphohydrolases, including phosphomono-, phosphodiesterases, and guanyl-specific ribonucleases which cleave RNA molecules to nucleoside-3'-phosphatases.
Autor:
M R, Sharipova, E V, Shakirov, N P, Balaban, L A, Gabdrakhmanova, M A, Shilova, G N, Rudenskaia, I B, Leshchinskaia
Publikováno v:
Mikrobiologiia. 69(5)
The activities of proteinases in the culture fluid and cellular fractions of Bacillus intermedius 3-19 grown under various conditions were studied. Thiol-dependent serine proteinase was the prevalent enzyme in the total pool of extracellular proteina
Autor:
L A, Gabdrakhmanova, E V, Shakirov, N P, Balaban, M R, Sharipova, G N, Rudenskaia, S V, Kostrov, T V, Akimkina, I B, Leshchinskaia
Publikováno v:
Mikrobiologiia. 69(5)
A nutrient medium was elaborated for the efficient production of glutamyl endopeptidase by the recombinant Bacillus subtilis strain AJ73 bearing the Bacillus intermedius 3-19 glutamyl endopeptidase gene within a multicopy plasmid. Optimal concentrati
Publikováno v:
Bioorganicheskaia khimiia. 26(9)
The gene for extracellular guanyl-specific ribonuclease of Bacillus thuringiensis var. subtoxicus (RNase Bth), a close homologue of the B. intermedius RNase (binase), was completely sequenced. Analysis of nucleotide sequences in the regions adjoining
Publikováno v:
Mikrobiologiia. 69(4)
Bacillus intermedius RNase added at a low concentration (0.001 microgram/ml) stimulated yeast growth, while a high RNase concentration (1500 micrograms/ml) was inhibitory to yeast growth. The inhibitory effect of RNase was transient and correlated wi