Zobrazeno 1 - 10
of 12
pro vyhledávání: '"I A, Pyshnaia"'
Autor:
A E, Karelov, I V, Pyshnaia, M V, Mitrokhina, I V, Popel', L V, Gorokhov, E G, Kremzel', E V, Naperov, A A, Nikolaev
Publikováno v:
Eksperimental'naia i klinicheskaia farmakologiia. 76(7)
The study of the efficiency and safety of the hepatotropic drug Remaxol in oncological patients with minimal, subclinical manifestations of liver dysfunction in the postoperative period was performed. It is shown that using of Remaxolcontributes to l
Publikováno v:
Bioorganicheskaia khimiia. 38(3)
The method of single nucleotide polymorphism identification based on primer extension reaction (PEXT) with the following bioluminescent solid-phase microassay was developed. The recombinant Ca2+-regulated photoprotein obelin and coelenterazine-depend
Autor:
A I, Zakabunin, T P, Kamynina, S N, Khodyreva, I A, Pyshnaia, D V, Pushnyĭ, E A, Khrapova, M L, Filipenko
Publikováno v:
Molekuliarnaia biologiia. 45(2)
The genes encoding of DNA ligases from the thermophilic archaeon Pyrococcus abyssi (PabDNA ligase) and Methanobacterium thermoautotrophicum (MthDNA ligase) were cloned and expressed in Escherichia coli. The activity of purified enzymes was studied by
Publikováno v:
Bioorganicheskaia khimiia. 36(5)
The features of UV-induced immobilization of oligonucleotides on a nylon membranes and the effectiveness of enzymatic labeling of immobilized probes at heterophase detection of nucleic acids are studied. Short terminal oligothymidilate (up to 10 nt)
Publikováno v:
Molekuliarnaia biologiia. 41(1)
The influence of fragmentation of analyzed DNA amplicon on the efficacy of specific sequence detection by means of heterophase hybridization analysis was investigated. The detection of DNA sequence was carried out colorimetrically after introduction
Publikováno v:
Zhurnal mikrobiologii, epidemiologii i immunobiologii. (3)
To determine DNA of herpes simplex virus (HSV), types 1 and 2, the polymerase chain reaction (PCR) method was developed with the subsequent detection of amplification products by means of electrophoresis or the molecular hybridization of nucleic acid
Autor:
P E, Vorob'ev, I A, Pyshnaia, D V, Pyshnyĭ, M N, Repkova, A G, Ven'iaminov, M A, Zenkova, E M, Ivanova, C, Scalfi-Happ, H, Seliger, G, Bonora, V F, Zarytova
Publikováno v:
Bioorganicheskaia khimiia. 26(11)
The 20-mer bridged oligodeoxynucleotides containing short oligomers joined by the hexamethylenediol and hexaethylene glycol linkers were shown to form complementary DNA/DNA and RNA/DNA complexes whose thermostability depends on the length and number
Autor:
D V, Pyshnyĭ, L M, Skobel'tsyna, E N, Gushchina, I A, Pyshnaia, I G, Shishkina, G M, Dymshits, V F, Zarytova, E M, Ivanova
Publikováno v:
Molekuliarnaia biologiia. 34(6)
Autor:
O A, Pliasunova, O A, Mamaeva, D V, Pyshnyĭ, I A, Pyshnaia, E M, Ivanova, P E, Vorob'ev, V F, Zarytova, A G, Pokrovskiĭ
Publikováno v:
Molekuliarnaia biologiia. 32(6)
Publikováno v:
Bioorganicheskaia khimiia. 23(11)
It was demonstrated that any mismatches in a complex formed by an ssDNA target and a tetranucleotide at 25 or 37 degrees C can be discriminated by alkylating the DNA with a tetranucleotide carrying a 4-[N-methyl-N-(2-chloroethyl)]aminobenzylethylamin