Zobrazeno 1 - 10
of 50
pro vyhledávání: '"I, Steuden"'
Publikováno v:
Glycoconjugate journal. 9(3)
Human erythrocyte glycophorin was desialylated by mild acid hydrolysis and degalactosylated by Smith degradation. Two monoclonal antibodies (Tn5 and Tn56) obtained by immunization of mice with this 'artificial' Tn antigen were characterized and compa
Autor:
Czeslaw Radzikowski, I. Steuden, Danuta Konopińska, Elwira Lisowska, Maria Duk, Kazimiera Waśniowska, Hubert Bartosz-Bechowski, Marcin Czerwinski, Danuta Duś
Publikováno v:
Molecular immunology. 29(6)
Analysis of epitopes for the three monoclonal antibodies (GPA105, GPA33, OSK4-1) against glycophorin A (GPA) was performed with the use of proteolytic fragments of GPA, the synthetic nonapeptide with the sequence of amino acid residues 35–43 of GPA
Publikováno v:
Glycoconjugate Journal. 2:303-314
The monoclonal antibody 22.19 of IgM class obtained after immunization of BALB/c mice with asialoglycophorin of human erythrocyte membranes is described. The specificity of this antibody for β-d-Gal-1-3-α-d-GalNAc- disaccharide chains (Thomsen-Frie
Publikováno v:
Anticancer research. 9(1)
The Thomsen-Friedenreich (TF) antigen is a cryptic disaccharide structure on human erythrocytes which can be exposed by neuraminidase treatment and which is supposed to be expressed in an unmasked form on some carcinoma cells. For its detection in ad
Autor:
P, Kuśnierczyk, A, Czarnomska, A, Opolski, M, Seshadri, E, Zborowska, C, Radzikowski, P, Kisielow, E, Pajtasz, I, Steuden
Publikováno v:
Archivum immunologiae et therapiae experimentalis. 35(4)
Seven inbred mouse strains, AKR, CBA, C3H, C57BL/6, C57BR/cd, DBA/2 and Swiss, maintained at the Bhabha Atomic Research Centre, Bombay (designated Bh) were monitored for compatibility with standard strains as well as for genetical homogeneity. For th
Publikováno v:
Hybridoma. 8(5)
Three monoclonal antibodies: 2.36.71.41, 7.60.66.55, and 18.4.40. 80 to human haptoglobin 2-1 were produced, purified and characterized. The affinity constants ranged within 0.3-2.4 x 10(8) M-1. The monoclonal antibodies 7.60.66.55 and 18.4.40.80 rea
Autor:
Włodzimierz Ostrowski, I. Steuden, Ewa Wasylewska, Jerzy Szkudlarek, Radosława Kuciel, Izydor Apostoł
Publikováno v:
The International journal of biochemistry. 18(11)
1. 1. IgG1 monoclonal antibody to purified seminal fluid phosphatase was raised by fusion of spleen cells from immunized mice with cell line Sp2/O-Ag 14 using simple method of screening for antiphosphatase antibody secreting clones. 2. 2. All molecul
Publikováno v:
Archivum immunologiae et therapiae experimentalis. 32(6)
Conventional antisera to L-1210 leukemia were being prepared in our laboratory for nearly a decade and consistently the only specificity detectable was anti Mammary Leukemia antigen (ML). Serological analysis of five monoclonal antibodies obtained fo
Characterization of anti-prostatic acid phosphatase monoclonal antibody and its medical significance
Autor:
R, Kuciel, A, Mazurkiewicz, W S, Ostrowski, J, Stachura, I, Steuden, J, Szkudlarek, C, Radzikowski
Publikováno v:
Biotechnology and applied biochemistry. 10(3)
Monoclonal antibody to purified prostatic acid phosphatase from seminal plasma was produced by fusion of spleen cells from immunized mice with the Sp2/O-Ag 14 cell line. This hybridoma-derived antibody, designated MAb-14, was classified as IgG1 immun
Publikováno v:
Archivum immunologiae et therapiae experimentalis. 36(5)
Two monoclonal antibodies, N/61 (IgG2b) and N/92 (IgG2a), reacting preferentially with blood group N antigen, were obtained by immunization of BALB/c mice with human red cell membrane glycophorins. The antibodies showed distinctly different specifici