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pro vyhledávání: '"Hyong-Bom Pak"'
Publikováno v:
Plant Biotechnology Reports. 14:255-262
Plasmid pCAMBIA2201-pta, containing the selectable marker neomycin phosphotransferase gene (npt II), Pinellia ternata agglutinin (PTA) gene and β-glucuronidase gene, were transformed into leaf discs of Chrysanthemum (Chrysanthemum morifolium Ramat.)
Autor:
Yong-Chol Pak, Song-Hui Pak, Hyong-Bom Pak, Sung-Ryong Ra, Myong-Suk Kim, Chon-Il Paek, Kum-Chol Ri
Publikováno v:
Folia Microbiologica. 64:803-808
Bacillus circulans 528 produces a restriction endonuclease, Bci528I which is an isoschizomer of EcoRI. We purified the enzyme, using Sephadex G-150, Phospho-cellulose, DEAE-cellulose, Hepharin-Sepharose CL-6B chromatography. The specific activity of