Zobrazeno 1 - 10
of 21
pro vyhledávání: '"Hye-Shin Chung"'
Publikováno v:
PLoS ONE, Vol 12, Iss 9, p e0185074 (2017)
Alpha 1-antitrypsin (A1AT) is a serine protease inhibitor that mainly inhibits neutrophil elastase in the lungs. A variant of A1AT at the P1 position with methionine 358 to arginine (A1AT-Pittsburgh) is a rapid inhibitor of thrombin with greatly dimi
Externí odkaz:
https://doaj.org/article/263f256a148d44738e3625d91ad058e9
Publikováno v:
PLoS ONE, Vol 11, Iss 7, p e0159451 (2016)
Here, we demonstrate that a metal ion binding motif could serve as an efficient and robust tool for site-specific conjugation strategy. Cysteine-containing metal binding motifs were constructed as single repeat or tandem repeat peptides and their met
Externí odkaz:
https://doaj.org/article/40a868a6e5e44187b2c48215e00de12c
Publikováno v:
Journal of Molecular Catalysis B: Enzymatic. 131:73-78
Organic solvent-tolerant enzymes are generally stable in organic solvents, but the conformational changes of the enzymes in organic solvents have not been well studied. As cold-adapted enzymes exhibit increased conformational flexibility as compared
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8f0ded8ef3144a8af9a0c9bb9ddc55b7
https://doi.org/10.1016/j.molcatb.2016.06.003
https://doi.org/10.1016/j.molcatb.2016.06.003
Publikováno v:
Glycoconjugate Journal. 33:201-208
Glycosylation affects the circulatory half-lives of therapeutic proteins. However, the effects of an additional N-glycosylation in the unstructured region or the loop region of alpha-1 antitrypsin (A1AT) on the circulatory half-life of the protein ar
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bae2b28cc400d27888f65b9a65bb1152
https://doi.org/10.1007/s10719-016-9657-3
https://doi.org/10.1007/s10719-016-9657-3
Publikováno v:
Extremophiles. 20:187-193
An aromatic amino acid, Tyr or Trp, located in the esterase active site wall, is highly conserved, with hyperthermophilic esterases showing preference for Tyr and lower temperature esterases showing preference for Trp. In this study, we investigated
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::757bdb75472ea40b976d648cd544b05d
https://doi.org/10.1007/s00792-016-0812-3
https://doi.org/10.1007/s00792-016-0812-3
Autor:
Hye-Shin Chung, Yeon Hee Park, Sunbae Lee, Seol-Hee Kim, Ji-Yeon Kim, Hee Kyung Ahn, Young-Hyuck Im, Jin Seok Ahn, Soon Jae Park
Publikováno v:
Journal of Clinical Oncology. 38:3551-3551
3551 Background: ALT-P7 is an antibody-drug conjugate, in which two molecules of monomethyl auristatin E (MMAE) are site-specifically conjugated to a cysteine-containing peptide motif of trastuzumab variant. This is the first-in-human study evaluatin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::2d063890367258860332f123a1f22f32
https://doi.org/10.1200/jco.2020.38.15_suppl.3551
https://doi.org/10.1200/jco.2020.38.15_suppl.3551
Publikováno v:
BMB Reports
Glucagon like peptide-1 (GLP-1) regulates glucose mediated-insulin secretion, nutrient accumulation, and β-cell growth. Despite the potential therapeutic usage for type 2 diabetes (T2D), GLP-1 has a short half-life in vivo (t1/2 ＜2 min). In an att
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ab86e53c29dd9c3b3e666697b1365455
https://doi.org/10.5483/bmbrep.2013.46.12.106
https://doi.org/10.5483/bmbrep.2013.46.12.106
Publikováno v:
Bioresource Technology. 148:620-623
In this study, the effect of graphene oxide (GO) on the thermal stability of a recombinant esterase from cold-adapted Pseudomonas mandelii, rEstKp, was investigated. The complex GO-rEstKp was formed by cross-linking. Both free rEstKp and GO-rEstKp co
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ba98ed6d0d7827df9bbfd890d07e1215
https://doi.org/10.1016/j.biortech.2013.09.018
https://doi.org/10.1016/j.biortech.2013.09.018
Publikováno v:
BMB Reports. 44:458-461
Enteropeptidase is a serine protease secreted by the pancreas and converts inactive trypsinogen to active trypsin. Enteropeptidase cleaves the C-terminal end of the substrate recognition sequence Asp-Asp-Asp-Asp-Lys (D(4)K). The assay for enteropepti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e2b7a1f32d1c98c378c54366ca5db725
https://doi.org/10.5483/bmbrep.2011.44.7.458
https://doi.org/10.5483/bmbrep.2011.44.7.458
Publikováno v:
Bulletin of the Korean Chemical Society. 30:2471-2474
Expression and purification of GLP-1/IgG-Fc fusion protein.(A) GLP-1/IgG-Fc fusion proteins were expressed in CHO-K1 cells. The 3, 6, 10-days conditioned medium were pooled and purified using a 5-mL HisTrap column on FPLC. Proteins separated by SDS-
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::41886b54ae2f1eea9c77f3fd4b45edcc
https://doi.org/10.5012/bkcs.2009.30.10.2471
https://doi.org/10.5012/bkcs.2009.30.10.2471